This preliminary study was designed to determine whether biologic action of biosynthetic human insulin (BHI) in vitro and its antigenicity in man equals that of various insulins of pancreatic origin. Testing the biologic action of BHI, pancreatic human insulin (PHI), and pancreatic pork insulin (PCPI) on glucose uptake by the rat hemidiaphragm and adipose tissue, identical efficacy for the insulins on a unit per unit basis was observed. Furthermore, a comparable impairment of insulin action was seen under the detrimental conditions of a hyperosmolal state. In an insulin-dependent diabetic female patient with cutaneous insulin hypersensitivity, BHI displayed the same antigenicity as PHI and PCPI. From these data we conclude tht BHI, both in vitro and in vivo, exhibits the same biologic properties as pancreatic insulin, and that it is reasonable to continue extensive testing of BHI in man, since identical metabolic effects and immunologic reactions are to be anticipated as with purified pancreatic insulin preparations of human or animal origin.
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Original Articles|
March 01 1981
Studies on the Biologic Actions of Biosynthetic Human Insulin In Vitro and in Diabetic Man
Werner K Waldhäusl;
Werner K Waldhäusl
Division of Clinical Endocrinology and Diabetes Mellitus, 1. Medizinische Universitätsklinik
Wien, Austria
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Gerhard Kastner;
Gerhard Kastner
Division of Clinical Endocrinology and Diabetes Mellitus, 1. Medizinische Universitätsklinik
Wien, Austria
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Martin Komjati;
Martin Komjati
Division of Clinical Endocrinology and Diabetes Mellitus, 1. Medizinische Universitätsklinik
Wien, Austria
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Paul Bratusch-Marrain
Paul Bratusch-Marrain
Division of Clinical Endocrinology and Diabetes Mellitus, 1. Medizinische Universitätsklinik
Wien, Austria
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Address reprint requests to W. Waldhäusl, Division of Clinical Endocrinology and Diabetes Mellitus, 1. Medizinische Universitätsklinik, Lazarettgasse 14, A-1090 Wien, Austria.
Citation
Werner K Waldhäusl, Gerhard Kastner, Martin Komjati, Paul Bratusch-Marrain; Studies on the Biologic Actions of Biosynthetic Human Insulin In Vitro and in Diabetic Man. Diabetes Care 1 March 1981; 4 (2): 205–208. https://doi.org/10.2337/diacare.4.2.205
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