Biosynthetic human insulin (BHI) obtained from separately synthesized A- and B-chains by recombinant DNA technology with Escherichia coli fermentation was compared with human and pork insulin of high purity in vitro. Applying four biologic tests (glucose oxidation and glucose incorporation into the lipids by rat epididymal fat pads, inhibition of lipolysis, and ATP depletion of isolated fat cells) and three receptor assays (binding competition with human fat cells, IM-9 lymphocytes, and rat liver cell plasma membranes), we could not discern significant differences of the half-maximum response by these seven methods. The only variance occurred with the ATP-depletion assay. This method disclosed 10% greater maximum reversion of isoproterenol-induced ATP depletion by BHI when compared with pork insulin.
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Original Articles|
March 01 1981
Potency of Biosynthetic Human Insulin Determined In Vitro
R D Fussgaenger;
R D Fussgaenger
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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H H Ditschuneit;
H H Ditschuneit
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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H Martini;
H Martini
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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H Wiebauer-De Lenardis;
H Wiebauer-De Lenardis
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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H Etzrodt;
H Etzrodt
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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Ch Thun;
Ch Thun
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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H Ditschuneit;
H Ditschuneit
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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E F Pfeiffer;
E F Pfeiffer
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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F Enzmann
F Enzmann
Departments of Internal Medicine II and I, University of Ulm
Germany, and Eli Lilly Co, Bad Homburg, Germany
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Address reprint requests to R.D. Fussgaenger, Department of Internal Medicine, University of Ulm, Steinhovelstrasse 9, 7900, Ulm, Germany.
Citation
R D Fussgaenger, H H Ditschuneit, H Martini, H Wiebauer-De Lenardis, H Etzrodt, Ch Thun, H Ditschuneit, E F Pfeiffer, F Enzmann; Potency of Biosynthetic Human Insulin Determined In Vitro. Diabetes Care 1 March 1981; 4 (2): 228–234. https://doi.org/10.2337/diacare.4.2.228
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