Biosynthetic human insulin (BHI) and pancreatic human insulin were compared with respect to receptor binding in a heterologous assay system: displacement of pork A14-125I-monoiodoinsulin from receptors on pig hepatocytes. The concentrations of human insulin giving half-maximal displacement were identical for both preparations, i.e., 0.5 nM. Their relative potency was 1.01 ± 0.14 (SD, N = 5), suggesting that biosynthetic and pancreatic human insulin exert the same biologic activity.
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Original Articles| March 01 1981
Receptor Binding of Biosynthetic Human Insulin on Isolated Pig Hepatocytes
Address reprint requests to S. Gammeltoft, Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK-2100, Copenhagen, Denmark.
Steen Gammeltoft; Receptor Binding of Biosynthetic Human Insulin on Isolated Pig Hepatocytes. Diabetes Care 1 March 1981; 4 (2): 235–237. https://doi.org/10.2337/diacare.4.2.235
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