Growth arrest–specific 6 (GAS6) is a secreted protein that acts as a ligand for TAM receptors (TYRO3, AXL, and MERTK). In humans, GAS6 circulating levels and genetic variations in GAS6 are associated with hyperglycemia and increased risk of type 2 diabetes. However, the mechanisms by which GAS6 influences glucose metabolism are not understood. Here, we show that Gas6 deficiency in mice increases insulin sensitivity and protects from diet-induced insulin resistance. Conversely, increasing GAS6 circulating levels is sufficient to reduce insulin sensitivity in vivo. GAS6 inhibits the activation of the insulin receptor (IR) and reduces insulin response in muscle cells in vitro and in vivo. Mechanistically, AXL and IR form a complex, while GAS6 reprograms signaling pathways downstream of IR. This results in increased IR endocytosis following insulin treatment. This study contributes to a better understanding of the cellular and molecular mechanisms by which GAS6 and AXL influence insulin sensitivity.

Article Highlights
  • Growth arrest–specific 6 (GAS6)–deficient mice are characterized by improved glucose tolerance and increased insulin sensitivity and are protected from diet-induced insulin resistance.

  • The GAS6 receptor AXL is expressed in muscle cells and forms a complex with the insulin receptor.

  • GAS6 reprograms insulin signaling by modulating the phosphorylation of proteins implicated in endocytosis, vesicle-mediated transport, and membrane trafficking.

  • AXL and GAS6 promote insulin receptor endocytosis following insulin treatment.

This article contains supplementary material online at https://doi.org/10.2337/figshare.26316043.

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