Pancreatic islets from BALB/c (H-2d) mice are rejected within 14 days of transplantation to the kidney capsule of allogeneic, CBA/H (H-2k) recipients. Cyclophosphamide pretreatment of the islet donor reduced the intensity of the allograft response, and these islets undergo a more chronic rejection process. Islets from cyclophosphamide-pretreated donors can be cultured in a gas phase of 95% O2 and 5% CO2, provided the islets are aggregated into clusters of about 50 islets. After a culture period of 7–12 days, the islet tissue was transplanted to normal allogeneic recipients, where the tissue elicited little or no allograft response over a 3-mo observation period.
Successful Allotransplantation of Mouse Pancreatic Islets to Nonimmunosuppressed Recipients
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Kerry M Bowen, Linda Andrus, Kevin J Lafferty; Successful Allotransplantation of Mouse Pancreatic Islets to Nonimmunosuppressed Recipients. Diabetes 1 February 1980; 29 (Supplement_1): 98–104. https://doi.org/10.2337/diab.29.1.S98
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