Interferon-γ Induces Class II MHC Antigens on RINm5F Cells

The ability of recombinant interferon-γ (rIFN-γ) to induce major histocompatibility complex (MHC) antigen expression in the rat insulinoma cell line RINm5F was investigated. The cells were stained with monoclonal antibodies specific for rat class I and class II MHC antigens. RINm5F cells endogenously expressed class I antigens; this was enhanced by rIFN-γ. Class II antigens could not be detected on RINm5F cells, but both I-A and I-E were induced by rIFN-γ.