We quantified fetal rat extrapancreatic insulin-gene expression by measuring mRNA in the yolk sac and placenta. Yolk sac makes a significant contribution to the total fetal insulin stores. The placenta contains a much smaller amount of insulin mRNA. Yolk sac insulin mRNA is barely detectable at 16 days gestation but increases markedly to a maximum at 21 days, 1 day before birth. In contrast to the pancreatic 550-nucleotlde (n) insulin mRNA, yolk sac has a 720-n mRNA. However, on removal of the terminal poly(A), both transcripts produce a 440-n RNA, the size predicted for a fully processed insulin mRNA.
Original Articles| January 01 1989
Insulin-Gene Expression in Extrafetal Membranes of Rats
Address correspondence and reprint requests to Dr. Joseph Locker, University of Pittsburgh, School of Medicine, Department of Pathology, Scaife Hall, Room 781, Pittsburgh, PA 15261.
Kimberley Rau, Louis Muglia, Joseph Locker; Insulin-Gene Expression in Extrafetal Membranes of Rats. Diabetes 1 January 1989; 38 (1): 39–43. https://doi.org/10.2337/diab.38.1.39
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