Intraportal islet autografting can restore near-normal glucose homeostasis in large diabetic animals, but the long-term failure rate of such grafts remains high. To assess the effect of the site of transplantation, we compared the hormonal responses to glucose (500 mg/kg i.v.) of intraportal (IP) and intrasplenic (IS) islet autografts in the cynomolgus monkey previously rendered diabetic by total pancreatectomy. Intravenous glucose tolerance tests (IVGTTs) 6 wk after IP grafting (n = 10) demonstrated nearly normal plasma glucose changes, with qualitatively normal but quantitatively reduced insulin and glucagon responses; only two animals have maintained these responses for >2 yr. IVGTTs 6 wk after IS grafting (n = 4) demonstrated more abnormal plasma glucose changes, with qualitatively normal but weak insulin responses and glucagon levels that did not fall in response to hyperglycemie; only one animal has maintained fasting normoglycemia for >9 mo. These results suggest that IS transplantation confers no benefit over IP transplantation in this model.
Metabolic Function of Intraportal and Intrasplenic Islet Autografts in Cynomolgus Monkeys
Robert Sutton, Derek WR Gray, Melanie Burnett, Philip McShane, Robert C Turner, Peter J Morris; Metabolic Function of Intraportal and Intrasplenic Islet Autografts in Cynomolgus Monkeys. Diabetes 1 January 1989; 38 (Supplement_1): 182–184. https://doi.org/10.2337/diab.38.1.S182
Download citation file: