Nonobese diabetic (NOD) mice and β2-microglobulin-gene–ablated mice (β2M –/–) show impaired presentation of major histocompatibility complex (MHC) class I and self-peptides, structures now recognized as critical for T-cell education to endogenous peptides. The naturally occurring NOD class I presentation abnormality appears to be attributable to, in part, a quantitative defect in the production of Tap-1 mRNA; Tap-1 with Tap-2 normally functions as a transporter for stable self-peptide and class I assembly. This study attempts to reverse NOD and β2M –/– mouse autoreactivity by introduced or reestablished syngeneic class I presentation. Introduction of MHC class I and self-peptides on syngeneic MHC class I-matched cells specifically prevented diabetes in NOD mice and eliminated in vitro class I–directed T-cell autoreactivity in NOD and β2M –/– mice. Reestablishment of endogenousclass I and self-peptide presentation in NOD mice was achieved with two well-described cures for the NOD mouse, complete Freund's adjuvant and mouse hepatitis virus. Both treatments induced Tap-1 mRNA, reestablished class I presentation of endogenous antigens, and eliminated in vitro and in vivo T-cell autoreactivity of self-peptides in the class I groove. These results substantiate a therapeutic role of self-peptide complexed with class I for T-cell education and suggest that some well-described NOD treatments may work, in part, through reestablishment of tolerance through class I and self-peptide.
Elimination of Self-Peptide Major Histocompatibility Complex Class I Reactivity in NOD and β2-Microglobulin-Negative Mice
Rong Huang, Jane Guo, Xiangping Li, Denise L Faustman; Elimination of Self-Peptide Major Histocompatibility Complex Class I Reactivity in NOD and β2-Microglobulin-Negative Mice. Diabetes 1 September 1995; 44 (9): 1114–1120. https://doi.org/10.2337/diab.44.9.1114
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