OBJECTIVE—IGF-1 and the IGF-1 receptor (IGF-1R) have been implicated in the regulation of adipocyte differentiation and lipid accumulation in vitro.
RESEARCH DESIGN AND METHODS—To investigate the role of IGF-1 receptor in vivo, we have inactivated the Igf-1r gene in adipose tissue (IGF-1RaP2Cre mice) using conditional gene targeting strategies.
RESULTS—Conditional IGF-1R inactivation resulted in increased adipose tissue mass with a predominantly increased lipid accumulation in epigonadal fat pads. However, insulin-stimulated glucose uptake into adipocytes was unaffected by the deletion of the IGF-1R. Surprisingly, IGF-1RaP2Cre mice exhibited markedly increased somatic growth in the presence of elevated IGF-1 serum concentrations, and IGF-1 mRNA expression was significantly increased in liver and adipose tissue. IGF-1 stimulation of wild-type adipocytes significantly decreased IGF-1 mRNA expression, whereas the opposite effect was observed in IGF-1R–deficient adipocytes.
CONCLUSIONS—IGF-1R signaling in adipocytes does not appear to be crucial for the development and differentiation of adipose tissue in vivo, but we identified a negative IGF-1R–mediated feedback mechanism of IGF-1 on its own gene expression in adipocytes, indicating an unexpected role for adipose tissue IGF-1 signaling in the regulation of IGF-1 serum concentrations in control of somatic growth.
Insulin and the structurally related IGFs provide essential signals for the control of embryonic and postnatal development (1) through binding of their respective tyrosine kinase receptors. Conventional gene targeting strategies used to abrogate insulin receptor and IGF-1 receptor (IGF-1R) signaling reveal severe phenotypes of both insulin receptor–and IGF-1R–deficient mice. While inactivating the insulin receptor results in embryonic lethality (2), mice with a whole-body inactivation of the Igf-1r gene die shortly after birth (3), whereas heterozygous Igf-1r knockout mice have increased longevity, most likely due to greater resistance to oxidative stress (4). These data point to a role of IGF-1R signaling in the regulation of lifespan. To discern the relevance of IGF-1R signaling in individual tissues, mice with tissue-specific inactivation of the IGF-1R have been created. Tissue-specific knockout of the Igf-1r gene in osteoblasts displayed significant defects in bone formation with low levels of osteoblastogenesis (5), revealing a function of the IGF system in formation and maintenance of bone tissue. Mice with simultaneous functional inactivation of both insulin and IGF-1Rs in skeletal muscle have a low muscle mass and display muscle hypoplasia (6), suggesting that IGF-1 is a vital regulator of muscle development. Conversely, muscle-specific overexpression of IGF-1R resulted in local myofiber hyperplasia (7). β-Cell–specific deletion of the IGF-1R did not alter islet development but led to hyperinsulinemia and glucose intolerance in βIGF-1RKO mice (8). This model suggests that tissue-specific alterations in the IGF-1 signaling might have secondary effects on the organism.
Until now, the role of IGF-1R signaling in white adipose tissue (WAT) has remained unclear. In 3T3-L1 adipocytes, IGF-1 is an essential regulator of differentiation (9), and it was recently shown that IGF-1 stimulates both cell growth and lipogenesis during differentiation of human mesenchymal stem cells into adipocytes in vitro (10). However, the physiological role of IGF-1R signaling in adipose tissue in vivo has not been systematically studied. To investigate the role of the IGF-1R in the development and metabolism of adipose tissue, we generated mice lacking the IGF-1R in adipose tissue (IGF-1RaP2Cre) using a conditional gene targeting approach based on the Cre recombinase. Subsequently, we characterized the consequences of IGF-1R deletion in adipose tissue on morphological and metabolic parameters of IGF-1RaP2Cre mice up to an age of 32 weeks.
RESEARCH DESIGN AND METHODS
Generation of IGF-1RaP2Cre mice.
IGF-1Rflox/wt mice were created by inserting loxP [locus of crossover (x) in bacteriophage P1] sites flanking exon 3 of the Igf-1r gene using conditional gene targeting strategies and maintained on a pure C57BL/6 genetic background. A targeting vector was constructed encompassing 4.5 kb of intron 2 of the murine Igf-1r gene, a loxP site, an FRT-flanked neomycin resistance gene, 600-bp fragment containing exon 3, a second loxP site, and 1.3 kb of intron 3. Cre-mediated recombination and subsequent excision of exon 3 of the IGF-1R results in a frame shift after 213 codons, with an appended sequence of 27 amino acids followed by a stop codon in exon 4. Gene targeting was performed in Bruce4 ES cells. For transfection, 1 × 107 ES cells were transfected with 40 μg DNA. G418/Ganciclovir double-resistant colonies were picked ∼9 days after transfection and expanded on 96-well tissue culture dishes. Genomic DNA was extracted from each clone and analyzed by Southern blot and PCR analysis. Recovery, microinjection, and transfer of 3.5 day postcoitum embryos were performed according to standard procedures. Chimeric animals (80–90% chimerism based on coat color) were bred with FlpE deleter on a C57BL/6 background. Genotyping was performed by PCR on DNA extracted from tail biopsies using customized primers: 5′-TCC CTC AGG CTT CAT CCG CAA-3′ (sense) and 5′-CTT CAG CTT TGC AGG TGC ACG-3′ (antisense). The targeting strategy is shown in Fig. 1A. Mice on a mixed (C57BL/6 × 129/Sv) genetic background carrying the aP2 Cre transgene were made by cloning a 1.4-kb SacI/SalI complementary DNA fragment encoding the Cre recombinase, modified by inclusion of a nuclear localization sequence and a consensus polyadenylation signal, immediately downstream of the 5.4-kb promoter/enhancer of fatty acid–binding protein aP2 (11).
IGF-1RaP2Cre were derived by crossing IGF-1Rflox/wt mice with IGF-1Rflox/wt mice expressing the Cre recombinase under the control of the aP2 promoter/enhancer (IGF-1RhetaP2Cre). All mice were housed in pathogen-free facilities in groups of three to five at 22 ± 2°C on a 12-h light/dark cycle. Animals were fed a standard chow diet (Altromin, Lage, Germany). In a subgroup of six to eight female IGF-1RAP2Cre and six wild-type mice, a high-fat diet study was performed with a special high-fat diet containing 55.2% of calories from fat (C1057; Altromin). Animals had ad libitum access to water at all times, and food was only withdrawn if required for an experiment. All experiments were performed in accordance with the rules for animal care of the local government authorities (Bezirksregierung Köln, Cologne, Germany) of the German Government and were approved by the institutional animal care and use committee.
Molecular characterization and genotyping of the IGF-1RaP2Cre mice.
Genotyping was performed by PCR using genomic DNA isolated from the tail tip. In brief, genomic DNA was prepared by using the DNeasy kit (Qiagen, Hilden, Germany). The following two primer pairs were used to genotype Igf-1r loxP sites, 5′-TCC CTC AGG CTT CAT CCG CAA-3′ (forward) and 5′-CTT CAG CTT TGC AGG TGC ACG-3′ (reverse), as well as the aP2 Cre recombinase 5′-CGC CGC ATA ACC AGT GAA AC-3′ (forward) and 5′-ATG TCC AAT TTA CTG ACC G-3′ (reverse). PCR was performed for 25 cycles (loxP sites) or 30 cycles (aP2 Cre) of 95°C, 59°C (loxP sites), or 58°C (aP2 Cre), and 72°C (30 s each) using the Qiagen Taq Polymerase and a Peltier Thermal Cycler PTC-200 (Bio-Rad, Hercules, CA). DNA from wild-type mice produced a 300-bp band, and a 380-bp band was detected in IGF-1R lox mice (Fig. 1B). For Western blot analysis, tissues were removed and homogenized in homogenization buffer with an ultra-Turrax homogenizer (IKA Werke, Stauffen, Germany), proteins were isolated using standard techniques, and Western blot analysis was performed with antibodies raised against IGF-1R β-subunit (Santa Cruz Biotechnology, C20) and β-actin (Abcam, Cambridge, U.K.) as loading control.
Phenotypic characterization.
Ten mice of each genotype (IGF-1RaP2Cre, IGF-1RhetaP2Cre, wild-type, IGF-1Rflox/flox, and aP2 Cre) of both sexes were studied from an age of 4 weeks up to 32 weeks of life. Body weight was recorded weekly up to an age of 14 weeks, and thereafter in 2-week intervals; body length (naso-anal length) was measured once at week 32. At an age of 16 weeks, both sexes of a subgroup of 20 (10 IGF-1RaP2Cre and 10 controls) underwent a food intake measurement over a time period of 1 week. The daily food intake was calculated as the average intake of chow within the time stated. Intraperitoneal glucose tolerance tests (GTTs) and insulin tolerance tests (ITTs) were performed at the age of 12 and 24 weeks. GTT was performed after an overnight fast for 16 h by injecting 2 g/kg body wt glucose and measuring the blood glucose levels after tail vein incision at 0 (baseline), 10, 30, 60, and 120 min after injection. ITT was performed in random-fed animals by injecting 0.75 unit/kg body wt human regular insulin (40 units Actrapid; Novo Nordisk, Copenhagen, Denmark). Glucose levels were determined in blood collected from the tail tip immediately before and 15, 30, and 60 min after the intraperitoneal injection.
Mice were killed at the age of 32 weeks by an overdose of anesthetic (Sevofluran, Abbott, Germany). Liver, heart, brain, lung, spleen, subcutaneous, and epigonadal adipose tissue were immediately removed. The organs were weighed and related to the whole-body mass to obtain relative organ weights. Serum was collected at 32 weeks, and concentrations of insulin, leptin, adiponectin, and IGF-1 were measured.
Analytical procedures.
Blood glucose values were determined from whole–venous blood samples using an automated glucose monitor (GlucoMen; Menarini Diagnostics, Wokingham, U.K.). Insulin, leptin, growth hormone (GH), and adiponectin serum concentrations were measured by ELISA using mouse standards according to the manufacturer's guidelines (Mouse/Rat Insulin ELISA; Linco, St. Charles, MO) (Mouse Leptin ELISA and Mouse Adiponectin/Acrp30 ELISA; R&D Systems, Minneapolis, MN). IGF-1 serum concentrations were measured by a radioimmunoassay (Mediagnost, Reutlingen, Germany).
Adipocyte isolation, size distribution, and primary culture.
Animals were killed, and subcutaneous and epididymal fat pads were removed. Adipocytes were isolated by 1 mg/ml collagenase digestion. To determine cell size distribution and adipocyte number, 200-μl aliquots of adipocytes were fixed with osmic acid, incubated for 48 h at 37°C, and counted in a Coulter counter (Multisizer III; Beckman Coulter, Krefeld, Germany). Isolated adipocytes were resuspended in Dulbecco's modified Eagle's medium/F-12 Ham's medium (Sigma, St. Louis, MO) and centrifuged at 350 × g for 10 min to separate mature adipocytes from the stromal vascular cell pellet, which was discarded. Floating fat-filled adipocytes were than incubated for 6 h with different concentrations (0, 0.1, 1, 10, and 100 ng/ml recombinant IGF-1 (U.S. Biological, Swampscott, MA).
Histology.
Tissue was fixed in 4% buffered formaldehyde, trimmed into small cubes, rinsed in phosphate-buffered saline, and dehydrated in a graded series of 50–100% ethanol followed by propylene oxide. Tissue was infiltrated with propylene:epon 812 mixtures by increasing the resin concentration gradually. Sections at 0.5 μm were cut with a Reichert ultramicrotome, mounted on polylysine-covered object slides and stained with azur-II-methylene blue (Richardson stain). Digitized pictures were taken with the light microscope Axioplan 2 (Zeiss, Jena, Germany) using the measurement facilities of Imagic Access (Imagic, Glattburg, Switzerland). Multiple sections (separated by 70–80 μm each) were obtained from gonadal and subcutaneous fat pads and analyzed systematically with respect to adipocyte size and number. For each genotype and sex, at least 10 fields (representing ∼100 adipocytes) per slide were analyzed.
Glucose transport.
For the determination of glucose transport, isolated adipocytes from the different fat depots were stimulated with 100 nmol/l insulin for 30 min and then incubated for 30 min with 3 μmol/l [U-14C]glucose. Immediately after incubation, adipocytes were fixed with osmic acid and incubated for 48 h at 37°C, and radioactivity was quantified after the cells had been decolorized (12).
Tissue-specific IGF-1 mRNA expression.
IGF-1 mRNA expression was measured by quantitative real-time PCR using the standard curve method in a fluorescent temperature cycler using the TaqMan assay. Fluorescence was detected on an ABI PRISM 7000 sequence detector (Applied Biosystems, Darmstadt, Germany). Total RNA was extracted from epigonadal and subcutaneous WAT, primary adipocyte culture after incubation with or without IGF-1, liver, brain, skeletal muscle, and brown adipose tissue (BAT) using TRIzol (Life Technologies, Grand Island, NY), and 1 μg RNA was reverse transcribed with standard reagents (Life Technologies, Grand Island, NY). From each RT-PCR, 2 μl was amplified in a 26-μl PCR using the Brilliant SYBR Green QPCR Core Reagent kit from Stratagene (La Jolla, CA) according to manufacturer's instructions. The following primer pairs were used: muIGF-1, 5′-GCTGCTGAAGCCATTCATTT-3′ (sense) and 5′-TTGCTCTTAAGGAGGCCAAA-3′ (antisense); mu36B4, 5′-aacatgctcaacatctcccc-3′ (sense) and 5′-ccgactcctccgactcttc-3′ (antisense). Expression of IGF-1 and 36B4 mRNA were quantified by using the second derivative maximum method of the TaqMan software (Applied Biosystems) determining the crossing points of individual samples by an algorithm that identifies the first turning point of the fluorescence curve. Amplification of specific transcripts was confirmed by melting curve profiles (cooling the sample to 68°C and heating slowly to 95°C with measurement of fluorescence) at the end of each PCR.
Data analysis and statistics.
Data are given as means ± SD. Datasets were analyzed for statistical significance using a two-tailed unpaired Student's t test, or differences were assessed by one-way ANOVA corrected by Bonferroni-Holm using the Statistical Package for Social Science, version 14.0 (SPSS, Chicago, IL). P values <0.05 were considered significant.
RESULTS
Generation of IGF-1RaP2Cre mice.
Mice lacking the IGF-1R in adipose tissue (IGF-1RaP2Cre) were generated by crossing mice carrying the loxP-flanked Igf-1r allele with transgenic mice expressing the Cre recombinase under control of the adipose-specific fatty acid binding protein (aP2) promoter. The targeting strategy is shown in Fig. 1A. IGF-1RaP2Cre mice were obtained with the expected Mendelian frequency. Cre expression was expected to be restricted to WAT and BAT. PCR analysis of the genomic DNA showed that the wild-type Igf-1r allele was absent in IGF-1RaP2Cre, whereas IGF-1RhetaP2Cre mice contained both the wild-type and loxP-flanked Igf-1r allele (Fig. 1B). Western blot analysis of adipose tissue lysates clearly indicated that Igf-1r protein was reduced by ∼90% in WAT of IGF-1RaP2Cre mice (Fig. 1C) and completely lost in BAT (data not shown). A ∼40% reduction of IGF-1R protein expression was found in the brain, whereas no changes in the overall expression of the IGF-1R protein were detected in liver, skeletal muscle (Fig. 1C), kidney, and bone (data not shown). IGF-1R expression was unaffected in all tissues of wild-type, IGF-1Rflox/flox, and aP2 Cre mice, indicating that neither the loxP modification of the IGF-1R locus nor expression of the aP2 transgene alone affects IGF-1R expression. These genotypes (wild type, IGF-1Rlox/lox, and aP2 Cre) had similar physiological and metabolic characteristics and were therefore considered controls in all subsequent analyses.
Phenotype of IGF-1RaP2Cre mice.
Both sexes of IGF-1RaP2Cre mice exhibited normal growth until the age of 10 weeks. However, by 10 weeks of age male and female IGF-1RaP2Cre mice had gained more weight than control group littermates (Fig. 2A and B). Moreover, we found that IGF-1RaP2Cre mice had significantly increased naso-anal body length when compared with littermate controls (females, P < 0.01; and males, P < 0.001) at an age of 24 and 32 weeks (Fig. 2C). Heterozygous deficiency of the Igf-1r gene had no significant influence on body growth and weight (data not shown). Daily food intake was indistinguishable between IGF-1RaP2Cre mice and all control groups (data not shown). Because IGF-1RaP2Cre mice exhibit ∼15% increased body weight, organ and fat pad weights were calculated as percentage of the whole-body weight at an age of 24 weeks. We found a disproportional increase of fat pad (Fig. 2D), liver (Fig. 2E), and heart weights in IGF-1RaP2Cre mice. In contrast, relative brain weight was significantly reduced in IGF-1RaP2Cre compared with controls (Fig. 2F). Relative organ weights of BAT (Fig. 2G), skeletal muscle, kidney, spleen, and bone were not different between IGF-1RaP2Cre mice and control mice (data not shown).
Metabolic parameters.
To determine the physiological consequences of reduced adipose tissue IGF-1R expression, we monitored blood glucose, insulin, total cholesterol, and triglyceride concentrations and performed serial glucose and ITTs over an age range from 12 to 32 weeks (Table 1). Fasted blood glucose was significantly higher in IGF-1RaP2Cre mice at an age of 12 weeks, whereas no such differences were found at 24 weeks of age (Table 1). In parallel, fed blood glucose was significantly higher at an age of 12 weeks in males (Table 1). There were no differences in serum insulin and leptin concentrations between IGF-1RaP2Cre and control mice at 12 and 24 weeks (Table 1). Cholesterol and triglyceride serum concentrations were also not significantly different between IGF-1RaP2Cre and control mice (Table 1). Adiponectin serum concentrations were higher in control compared with IGF-1RaP2Cre mice, however, these differences were only significant at 12 weeks (Table 1). Independent of age, intraperitoneal GTTs demonstrated normal glucose tolerance in male (Fig. 3A and B) and female (data not shown) IGF-1RaP2Cre and control mice. Intraperitoneal ITTs at 12 and 24 weeks of age in male (Fig. 3C and D) and female mice (data not shown) were indistinguishable between IGF-1RaP2Cre and control mice. Taken together, reducing IGF-1R expression in adipose tissue results in increased somatic growth in the absence of major metabolic alterations.
IGF-1 serum concentration is markedly increased in IGF-1RaP2Cre mice.
Because IGF-1RaP2Cre mice exhibited an increased postnatal somatic growth, we next determined circulating plasma IGF-1 concentrations as the major determinant of somatic growth in mammals. IGF-1 serum concentration is significantly increased in IGF-1RaP2Cre mice starting at an age of 12 weeks up to 24 weeks of age (Fig. 4A). In parallel to higher IGF-1 serum concentrations, we found significantly higher serum concentrations of the IGF binding protein (IGFBP)-3 (Fig. 4B). On the other hand, serum concentrations of IGFBP-1 and -2 were not significantly different between IGF-1RaP2Cre and control mice (data not shown). GH and IGF-2 serum concentrations were indistinguishable between IGF-1RaP2Cre and control mice (Table 1), indicating that dysregulation of IGF-1 concentrations is not a result of altered pituitary regulation and thus appears to be primarily regulated in peripheral organs of IGF-1RaP2Cre mice. Therefore, we determined IGF-1 and IGFBP-3 mRNA expression in adipose tissue and liver of IGF-1RaP2Cre and control mice to assess whether increased IGF-1 and IGFBP-3 serum concentrations correlate with increased expression in those organs important for the synthesis of these molecules. We found significantly higher IGF-1 mRNA expression both in adipose tissue (Fig. 4C) and liver (Fig. 4D) of IGF-1RaP2Cre mice. In parallel, there was a trend for increased IGFBP-3 mRNA expression in liver and adipose tissue of IGF-1RaP2Cre compared with control mice (data not shown).
To determine whether increased adipose tissue IGF-1 expression in IGF-1RaP2Cre mice was the result of an altered adipocyte autonomous autocrine regulatory loop, we isolated adipocytes from wild-type and IGF-1RaP2Cre mice. Treatment of control adipocytes with recombinant IGF-1 (100 ng/ml) for 6 h ex vivo caused a significant reduction of its own mRNA expression (Fig. 4E). Conversely, in adipocytes from IGF-1RaP2Cre mice, stimulation with IGF-1 did not inhibit IGF-1 mRNA expression but rather resulted in a paradoxical stimulation of IGF-1 mRNA expression.
Consequences of IGF-1R knockout on adipose tissue morphology and metabolism.
Adipose tissue mass is increased in IGF-1RaP2Cre mice compared with controls (Fig. 2D). The increased adipose tissue mass was related to both increased number and mean volume of adipocytes in IGF-1RaP2Cre mice (data not shown). Mean adipocyte size (diameter) is significantly higher in IGF-1RaP2Cre (105.4 ± 12 μm) when compared with control (87.3 ± 9.3 μm) mice (P < 0.05). These differences are more pronounced in epigonadal than in subcutaneous fat (Fig. 5A and B). We found a 1.4-fold increase in insulin receptor mRNA expression in adipocytes from IGF-1RaP2Cre mice (data not shown). To determine the consequences of IGF-1R knockout on adipocyte glucose transport, basal and insulin-stimulated glucose uptake in isolated adipocytes was studied. In adipocytes from IGF-1RaP2Cre mice, basal glucose uptake is unchanged compared with wild-type mice (Fig. 5C). Insulin stimulated glucose uptake is 2.8-fold in IGF-1RaP2Cre and 2.1-fold in wild-type adipocytes (P < 0.05). Despite larger adipocyte size in IGF-1RaP2Cre mice, we found significantly increased insulin sensitivity in IGF-1RaP2Cre compared with wild-type adipocytes.
DISCUSSION
IGFs (IGF-1 and -2) lead to many different biological effects in target cells such as hepatocytes, myocytes, and adipocytes (13). IGF-1 action is propagated by the IGF-1R. IGF-1R–deficient mice are severely growth retarded, exhibit multiple growth- and differentiation-dependent abnormalities, and die shortly after birth (3). Disruption of IGF-1R in specific tissues or cell types such osteoblasts (5), skeletal muscle (6), and β-cells (8) further revealed an essential role of IGF-1R signaling in differentiation and growth.
IGF-1 was shown to be an essential regulator of differentiation in 3T3L1 adipocytes (9). Moreover, IGF-1 stimulates both cell growth and lipogenesis during differentiation of human mesenchymal stem cells into adipocytes in vitro (10). However, the physiological role of the IGF-1R signaling in adipose tissue in vivo has not been systematically studied. We therefore created mice lacking the IGF-1R in adipose tissue (IGF-1RaP2Cre mice). To specifically target adipose tissue, we used transgenic mice that express the Cre recombinase cDNA from the adipose-specific fatty acid binding protein (aP2) promoter/enhancer (14). aP2 Cre mice have been used for adipose-selective inactivation of the GLUT4 and insulin receptor genes (11,15,16). Unexpectedly, deletion of IGF-1R was not restricted to WAT and BAT. In most IGF-1RaP2Cre mice, we found a reduction of IGF-1R protein also in the brain, suggesting transgene expression in neuronal tissue in addition to fat. Although aP2 promoter provides adipocyte-restricted expression postnatally, it was recently shown that aP2 has a wider embryonic expression pattern than previously appreciated (17). Throughout embryonic development, the aP2 Cre transgene was consistently found in different cell types of the nervous system (17). Expression of the aP2 Cre transgene during embryonic development might explain the reduced relative brain mass in IGF-1RaP2Cre mice. We excluded aP2 Cre transgene expression in other tissues, including liver and skeletal muscle, by protein expression analyses. Whole-body expression of the transgene would cause growth retardation as demonstrated in IGF-1r+/− mice (4,18). In contrast, IGF-1RaP2Cre mice have increased somatic growth, further suggesting that tissues other than fat and brain are not affected by the ectopic aP2-Cre transgene expression. However, we have to discuss our data with the caution that reduced IGF-1R expression in the brain may contribute to the phenotype of IGF-1RaP2Cre mice. IGF-1RaP2Cre mice are viable and fertile and do not exhibit growth retardation.
We demonstrate that IGF-1RaP2Cre mice have increased somatic growth starting at an age of 10 weeks. IGF-1RaP2Cre mice are both longer and heavier than their wild-type littermates. The increased growth is not the result of increased food intake in IGF-1RaP2Cre mice. Interestingly, adipose tissue, liver, and heart exhibit higher relative organ weights than other organs, including kidney, skeletal muscle, and spleen, which are increased in size proportionally to the higher body weight of IGF-1RaP2Cre mice. Increased growth in IGF-1RaP2Cre mice is most likely the result of an ∼20% increased IGF-1 serum concentrations. In patients with acromegaly, elevated circulating IGF-1 levels cause increased adult growth and, at least in some patients, may lead to enlarged organs, including hepatomegaly (19) and acromegalic cardiomyopathy (20). In analogy to the phenotype of IGF-1RaP2Cre mice, transgenic expression of human IGF-1 in mice causes a similar phenotype with 1.3-fold increased weight as a result of selective organomegaly without an apparent increase in skeletal growth (21). Moreover, liver-specific IGF-1 overexpression causes 50% increased serum IGF-I levels and enhanced somatic growth (22).
Because circulating GH and IGF-2 levels are normal, increased IGF-1 serum levels in IGF-1RaP2Cre mice are most likely the result of increased IGF-1 expression both in adipose tissue and liver and not of changes caused by an IGF-1R deficiency in the central nervous system. It is difficult to dissect whether adipose or liver-derived IGF-1 primarily increases circulating IGF-1 levels. Interestingly, we identified a negative feedback mechanism for IGF-1 mRNA expression in adipose tissue. In wild-type adipocytes, IGF-1 suppressed its own mRNA expression ex vivo, whereas this negative feedback was blunted in adipocytes isolated from IGF-1RaP2Cre mice. However, increased IGF-1 expression in liver is more difficult to explain.
It is difficult to dissect whether adipose or liver-derived IGF-1 primarily increases circulating IGF-1 levels. Our experimental design does not allow for the distinction between hepatic or adipose tissue–derived IGF-1. It has been shown that IGF-1 and IGF-1R mRNA levels are differentially regulated in different tissues (23). Interestingly, presumably adipose tissue–derived IGF-1 does not inhibit hepatic IGF-1 expression as it was shown in the liver of GH-deficient dwarf rats after IGF-1 treatment (24). Another potential explanation for the absence of a negative feedback of IGF-1 on its own expression in liver might be that hepatocytes only express the IGF-1R in very little amounts (25). Alternatively, circulating IGF-1 could be modulated indirectly by affecting its binding proteins. Increased IGFBP-3 mRNA expression in liver in response to increased circulating IGF-1 from adipose tissue could be a potential mediator. Increase in IGFBP-3 was the best and independent predictor of increased IGF-1 serum concentrations in our IGF-1RaP2Cre mice (27).
Moreover, deletion of the IGF-1 gene in liver demonstrated that liver-derived IGF-1 is not crucial for somatic growth (26), suggesting that increased somatic growth in IGF-1RaP2Cre mice is primarily due to increased adipose tissue expression. However, previous data suggest that systemic IGF-1 can feedback on hepatic IGF-1 mRNA expression in the GH-deficient state. Our IGF-1RaP2Cre mice have normal GH serum concentrations (Table 1), suggesting that under normal GH levels, hepatic IGF-1 expression does not depend on the same feedback mechanism. Moreover, we cannot explain why increased IGF-1 levels did not cause decreased GH serum concentrations. Therefore, we cannot exclude that reduction of IGF-1R receptor signaling in individual tissues causes a disruption of physiological feedback mechanisms in other systems or tissues. Adipose tissue–derived signals other than circulating IGF-1, GH, and/or IGF-2 might cause increased circulating IGF-1 levels and increased IGF-1 expression in liver and adipose tissue. Further studies are necessary to characterize these signals.
Circulating IGF-1 was shown to reduce IGF-1 mRNA expression in liver, whereas GH increases IGF-1 gene expression (23). In our model with normal GH but increased IGF-1 serum concentrations, one would expect decreased rather than the observed increased hepatic IGF-1 mRNA expression. One hypothesis is that lack of IGF-1R signaling in adipose tissue generates signals that indirectly increase both adipose and hepatic IGF-1 expression. However, GH and IGF-2 receptor expressions were indistinguishable between IGF-1RaP2Cre and control mice in both liver and adipose tissue, suggesting that no liver-specific compensatory mechanism accounts for the unsuppressed IGF-1 mRNA expression in liver. In parallel to increased IGF-1 serum concentrations, circulating IGFBP-3, but not IGFBP-1 and -2, was significantly increased in IGF-1RaP2Cre mice. Overexpression of human IGFBP-3 in transgenic mice results in selective organomegaly, including liver and heart (24), suggesting that increased IGFBP-3 levels contribute to organomegaly in IGF-1RaP2Cre mice.
Increased circulating IGF-1 by 50% was shown to improve glucose tolerance in mice with a liver-specific overexpression of the Igf-1 gene (22). Interestingly, we did not observe any improvement of glucose tolerance and insulin sensitivity in IGF-1RaP2Cre mice, suggesting that 20% increased IGF-1 levels are not sufficient to enhance whole-body insulin sensitivity. IGF-1RaP2Cre mice do not exhibit significant metabolic alterations. Interestingly, at the age of 12 weeks, when growth curves diverge, fasting blood glucose concentrations are significantly higher in IGF-1RaP2Cre than in controls. Because glucose tolerance is normal at this age, it is difficult to establish causality for elevated glucose concentrations. Higher adipose tissue mass could contribute to these metabolic alterations. We also found lower adiponectin serum concentrations in IGF-1RaP2Cre mice of both sexes. Lower circulating adiponectin levels further suggest that metabolic alterations in IGF-1RaP2Cre mice at 12 weeks of age are a consequence of increased fat mass. Serum adiponectin negatively correlates with fat mass in mice (16) and men (28). Insulin and IGF-1 are capable of binding to each other's receptor, although with different affinities (29). Mice with a targeted disruption of the insulin receptor in adipose tissue (FIRKO mice) are protected from obesity and its metabolic alterations (16). Moreover, these mice exhibit specific morphological changes in adipose tissue, which could be due to a defect in adipose tissue development. We therefore expected alterations in adipose tissue of IGF-1RaP2Cre, because IGF-1R was shown to be important in the growth and differentiation of adipocytes in vitro (9). Despite similarities in the structure and intracellular signaling between insulin and IGF-1Rs, we did not find a severe metabolic phenotype in mice lacking the IGF-1R in adipose tissue. However, we found significantly increased insulin-stimulated glucose uptake into isolated adipocytes from IGF-1RaP2Cre mice, suggesting increased glucose uptake and subsequent increase in lipogenesis as primary causes leading to increased lipid load per adipocyte and adipose tissue hypertrophy in IGF-1RaP2Cre mice.
This further suggests that insulin receptor signaling is more important for fuel metabolism, whereas IGF-1R mediates growth, and that one receptor cannot functionally compensate for the other receptor's absence (13).
Our data further show that IGF-1R signaling in adipocytes is not crucial for the growth, development, or differentiation of adipose tissue in vivo. Unexpectedly, both the number and the size of adipocytes is increased in adipose tissue of IGF-1RaP2Cre mice, either suggesting that intact IGF-1R is not necessary for adipose tissue development or that other mechanisms exist in vivo, which can compensate for the disruption of IGF-1R. In accordance with our results, it was recently shown that most IGF receptor–deficient adipocyte cell lines were capable of normal differentiation (13). Moreover, important markers of adipogenesis, including peroxisome proliferator–activated receptor-γ and GLUT4, were expressed despite the lack of IGF-1R (13). One potential mechanism could be that insulin receptor mediates the IGF-1 effects on growth and differentiation in vivo. This hypothesis is supported by increased insulin receptor gene expression in adipose tissue of IGF-1RaP2Cre mice. However, in vitro there was no compensation of insulin receptor when the IGF-1R was absent. Therefore, it appears that IGF-1R is not crucial for adipose tissue development. Interestingly, adipocytes from IGF-1RaP2Cre mice were more sensitive to insulin-stimulated glucose uptake than adipocytes from control mice. Our findings are in accordance with previously reported increased insulin sensitivity in IGF-1R–deficient brown adipocytes (30). Moreover, downregulation of IGF-1R in breast cancer cells was shown to increase insulin sensitivity (31). Diminished IGF-1R/insulin receptor hybrid receptor formation resulting in enhanced holo–insulin receptor formation could contribute to increased insulin sensitivity in IGF-1R–deficient adipocytes. In addition, increased insulin receptor number might enhance insulin effects on IGF-1RaP2Cre adipocytes.
In conclusion, IGF-1R signaling in adipocytes is not crucial for the development and differentiation of adipose tissue in vivo but seems to participate in the regulation of IGF-1 serum concentration.
Published ahead of print at http://diabetes.diabetesjournals.org on 28 April 2008.
N.K. and L.K. contributed equally to this work.
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Article Information
M.B. has received Deutsche Forschungsgemeinschaft Grant BL 580/3-1, Clinical Research group Atherobesity KFO 152 Project BL 833/1-1, and Project B24 from the Interdisciplinary Center of Clinical Research Leipzig at the Faculty of Medicine of the University of Leipzig.