In the article cited above, the authors did not list the description of the Western blot analysis in the research design and methods section and would like to add the following description:
Western Blot Analysis
Protein from ASCs was extracted in radioimmunoprecipitation assay buffer and subjected to Western blot analysis. The concentration of protein in lysates was determined using Bradford assay. Protein (20 µg) was separated on 4–20% Mini-PROTEAN gels (Bio-Rad) and then transferred to nitrocellulose membrane. The membranes were probed with primary antibodies, WT1 (1:1,000 dilution; Santa Cruz Biotechnology) and α-tubulin (1:5,000 dilution; Sigma). After subsequent washes and incubation with respective secondary antibodies, the horseradish peroxidase activity was detected using chemiluminescent reagents.
The online version reflects this change.
