Introduction & Objective: T2DM can be effectively treated by sleeve gastrectomy (SG), albeit the mechanisms underlying is elusive. Postoperative changes in serum bile acid have been found contributing to metabolism improvement. This study aims to determine the overall changes of bile acid enterohepatic circulation following SG.
Methods: SG model was established in T2DM rats. Alterations of bile acids in the bile, intestine, portal vein and inferior vena cava were evaluated by liquid chromatography-mass spectrometry. Bile acid transporters were also evaluated.
Results: Metabolism was significantly improved after SG. Intraluminal bile acids were altered from the duodenum to the colon, with glycine/taurine conjugated bile acid ratio being increased. Peripheral blood total bile acids exhibited an elevation, accompanied by intricate alterations in the composition of bile acids within both the bile and the portal vein. Postoperative liver bile acid absorption transporter NTCP, OATP and efflux transporter BSEP, MDR3 were significantly decreased, whereas intestinal uptake transporter ASBT was increased and bile acid binding protein IBABP was decreased (P<0.05 all).
Conclusion: The entire pathway of bile acid enterohepatic circulation exhibited complicated changes following SG. The impact of the changes on postoperative metabolism improvement warrants further research.
X. Zhao: None. Z. Liu: None. G. Yang: None. Z. Ge: None. S. Dong: None. X. Zhang: None. Z. Cheng: None. K. Wang: None.
National Natural Science Foundation of China (82070852, 82270901)
