An Abnormal Insulin in Juvenile Diabetes Mellitus

A technic is described for the isolation of insulin from serum using a series of gel nitrations in combination with an interim complexing with guinea pig anti-insulin serum. Insulin thus derived from the serum of juvenile diabetic subjects was shown to be significantly more resistant to insulinase than that from normal persons. It is suggested that there is a genetically determined structural difference in the insulin of diabetic children which contributes importantly to the underlying biochemical disorders of the disease.