The in vivo biologic activity of human serum bound insulin is significantly neutralized in vivo, in intact mice and rats, by undiluted guinea-pig insulin antisera (GPAIS). Significant neutralization of biologic activity on both muscle and adipose tissue glycogen synthesis was observed with the addition of GPAIS.

The present studies demonstrate that the concentration of insulin antisera is critical for the neutralization of in vivo biologic activity of bound insulin. In addition, the results suggest that bound insulin injected intraperitoneally into mice or rats may be modified in such a way to allow reactivity with insulin antisera, as judged by loss of biologic activity.

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