Isolated adipose tissue cells were prepared from subcutaneous samples obtained from nine morbidly obese subjects weighing from 187 to 306 per cent of ideal body weight. The responsiveness of these adipocytes to a number of test substances was determined by measuring cellular cyclic AMP concentration at one-half hour and glycerol release at four hours. Theophylline (10−3 M) and epinephrine (10−5 M) stimulated Upolysis; theophylline stimulated an increase in cyclic AMP, while epinephrine failed to prompt a significant change in the nucleotide. Neither the αblocker, phen tolamine (10−5 M), nor the β blocker, propranolol (3 × 10−5 M), affected lipolysis or cyclic AMP; when these agents were incubated in combination with epinephrine, changes occurred indicative of the presence of both α and β adrenergic receptor sites. Insulin significantly reduced both basal and stimulated lipolysis but failed to affect cyclic AMP. With minor exceptions, adipocytes from hyperobese. Subjects behaved similarly to cells from unselected donors; at the concentration used, there was no evidence of resistance to insulin.
Skip Nav Destination
Article navigation
Original Contributions|
July 01 1977
In-vitro Observations on Isolated Adipose Tissue Cells from Hyperobese Subjects
Thomas W Burns, MD;
Thomas W Burns, MD
Division of Endocrinology and Metabolism, Department of Medicine, Department of Surgery, University of Missouri Medical Center
Columbia, Missouri 65201
Program of Pharmacology, University of Texas Medical School
Houston, Texas 77025
Search for other works by this author on:
Boyd E Terry, MD;
Boyd E Terry, MD
Division of Endocrinology and Metabolism, Department of Medicine, Department of Surgery, University of Missouri Medical Center
Columbia, Missouri 65201
Program of Pharmacology, University of Texas Medical School
Houston, Texas 77025
Search for other works by this author on:
Paul E Langley;
Paul E Langley
Division of Endocrinology and Metabolism, Department of Medicine, Department of Surgery, University of Missouri Medical Center
Columbia, Missouri 65201
Program of Pharmacology, University of Texas Medical School
Houston, Texas 77025
Search for other works by this author on:
G Alan Robison, PhD
G Alan Robison, PhD
Division of Endocrinology and Metabolism, Department of Medicine, Department of Surgery, University of Missouri Medical Center
Columbia, Missouri 65201
Program of Pharmacology, University of Texas Medical School
Houston, Texas 77025
Search for other works by this author on:
Citation
Thomas W Burns, Boyd E Terry, Paul E Langley, G Alan Robison; In-vitro Observations on Isolated Adipose Tissue Cells from Hyperobese Subjects. Diabetes 1 July 1977; 26 (7): 657–662. https://doi.org/10.2337/diab.26.7.657
Download citation file:
37
Views