The extent of nonenzymatic glucosylation of serum protein in control and diabetic subjects was measured by a chemical procedure using thiobarbituric acid. A mean value of 0.81 (±0.21 SD) nmol glucose per milligram serum protein was observed in the control group. Diabetics displayed elevated levels of glucosylated serum proteins, up to 4 nmol glucose per milligram protein. Glucosylation of serum protein correlated strongly with fasting blood sugar (r = 0.71), percent hemoglobin A1 (r = 0.79), and percent glucosylated albumin (r = 0.99). There was no overlap between control and diabetic groups, i.e., within 3 SD of the mean of controls. These studies indicate that the assay for glucosylated serum protein appears to be an especially sensitive indicator of the degree of hyperglycemia in diabetes.
Nonenzymatic Glucosylation of Serum Proteins in Diabetes Mellitus
Kay F McFarland, Edward W Catalano, James F Day, Suzanne R Thorpe, John W Baynes; Nonenzymatic Glucosylation of Serum Proteins in Diabetes Mellitus. Diabetes 1 November 1979; 28 (11): 1011–1014. https://doi.org/10.2337/diab.28.11.1011
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