The purpose of this study was to determine if the somatostatin in pancreatic islets is synthesized via a precursor-product pathway. Anglerfish islets were subjected to pulse-chase incubations with 3H-tryptophan 14C-isoleucine. Islet extracts were subjected to gel filtration on Bio-Gel P-10. During chase incubations in the presence of cycloheximide, a linear increase of 3H-radioactivity in the somatostatin-containing portion of the eluate was observed while 14C-label did not accumulate in this region. A concomitant diminution of both 3H- and 14C- radioactivity in the 8-15-kilodalton region of the eluate occurred during the chase periods, suggesting a transfer of label from larger to smaller peptides. The labeled peptides found in the 8-15-kilodalton range were subjected to treatment with 8 M urea, 8 M urea−5% thioglycolic acid, 6 M guanidine hydrochloride, and 8 M guanidine hydrochloride−5% thioglycol. No appreciable loss of radioactivity was apparent after any of these treatments. Incubation of these same peptides with cell-free supernate from unlabeled islet tissue or with trypsin yielded a tryptophan-labeled peptide having the molecular size, electrophoretic mobility, and immunoreactivity of anglerfish and synthetic somatostatin. These results suggest that a precursor peptide having a molecular size near that of proinsulin is used in the biosynthesis of somatostatin.
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Original Contributions| August 01 1979
Evidence for the Existence of a Biosynthetic Precursor for Somatostatin
Bryan D Noe;
Donald J Fletcher;
Address reprint requests to B. D. Noe, Department of Anatomy, Emory University, Atlanta, Georgia 30322.
Bryan D Noe, Donald J Fletcher, Joachim Spiess; Evidence for the Existence of a Biosynthetic Precursor for Somatostatin. Diabetes 1 August 1979; 28 (8): 724–730. https://doi.org/10.2337/diab.28.8.724
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