The Friend erythroleukemia cell has an insulin receptor with all the properties of mammalian insulin receptors: rapid, reversible, and saturable binding of insulin; specific for insulin and insulin analogs; inversely proportional to temperatures; sharply pH dependent (optimum = 8.0); and demonstrated ligand-induced accelerated dissociation consistent with negative cooperativity. There were 17,200 sites per cell. After induction by dimethylsulfoxide, 80% of the cells became benzidine positive (i.e., contained hemoglobin). The receptor concentration dropped to 4300 sites per cell, while the remaining receptors retained all the initial binding characteristics. This loss of receptors could not be attributed directly to either dimethylsulfoxide or changes in cell size. Thus, during the process of differentiation, the concentration of insulin receptors in the Friend erythroleukemia cell decreases.
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Original Contributions|
September 01 1979
Decrease in Insulin Receptors During Friend Erythroleukemia Cell Differentiation
Barry H Ginsberg;
Barry H Ginsberg
Veterans Administration Hospital and The Diabetes-Endocrinology Research Center, the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa
Iowa City, Iowa 52240
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Thomas Brown;
Thomas Brown
Veterans Administration Hospital and The Diabetes-Endocrinology Research Center, the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa
Iowa City, Iowa 52240
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Mohan Raizada
Mohan Raizada
Veterans Administration Hospital and The Diabetes-Endocrinology Research Center, the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa
Iowa City, Iowa 52240
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Diabetes 1979;28(9):823–827
Article history
Received:
May 04 1979
Revision Received:
June 08 1979
PubMed:
288716
Citation
Barry H Ginsberg, Thomas Brown, Mohan Raizada; Decrease in Insulin Receptors During Friend Erythroleukemia Cell Differentiation. Diabetes 1 September 1979; 28 (9): 823–827. https://doi.org/10.2337/diab.28.9.823
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