Defective Regulation of Cl⁻ Permeability in Islets of Diabetic Mice [C57BL/KsJ(db/db)]

Efflux of ³⁶Cl⁻ from prelabeled, collagenase-isolated islets of noninbred ob/ob mice, inbred diabetic [C57BL/KsJ(db/db)] mice, and nondiabetic [C57BL/KsJ(+/+)] mice was studied by nonrecirculating perifusion. Islets of both ob/ob mice and nondiabetic KsJ mice showed similar rates of basal ³⁶Cl efflux, D-glucose stimulation of the ³⁶Cl⁻ efflux, and net uptake of ³⁶Cl⁻ at apparent isotope equilibrium. The ³⁶Cl⁻ efflux in islets from both young and old KsJ-db/db mice was almost insensitive to the D-glucose concentration. The basal rate of ³⁶Cl⁻ efflux in islets from young and old db/db mice was increased, indicating an abnormally high Cl⁻ permeability. It is suggested that the defective regulation of the membrane potential in B-cells from [C57BL/KsJ(db/db)] mice may at least partly be caused by a db-mediated defect in the regulation of Cl⁻ permeability.