The secretory responses of beta and delta cells were compared in the isolated, perfused, rat pancreas. Insulin release was stimulated 50-fold and somatostatin (SRIF) secretion twofold when the glucose concentration was increased from 100 mg/dl to 300 mg/dl. When islet cyclic AMP (cAMP) was raised by 3-isobutyl-1-methylxanthine (IBMX), the secretion of SRIF was stimulated in a glucose-dependent manner (300 mg/dl > 100 mg/dl > 25 mg/dl). As expected, insulin release was also potentiated in a similar fashion. A difference in the glucose dependency of the cAMP effect was seen at 25 mg/dl glucose, where 0.25 mM IBMX enhanced the release of SRIF but not that of insulin. In contrast, at 300 mg/dl glucose, IBMX caused a greater potentiation of insulin release than of SRIF release. The release of insulin, when expressed in absolute terms, was stimulated more markedly than that of SRIF under most conditions tested. However, the expression of total hormone released during 30 min of stimulation as a percentage of tissue hormone content allowed a different interpretation of the results. For example, 2.1% and 1.8% of tissue insulin and SRIF contents, respectively, was released during exposure to 300 mg/dl glucose. Surprisingly, under basal conditions (100 mg/dl glucose), 1.0% of total SRIF content was released during 30 min compared with the release Of only 0.04% of insulin.
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January 01 1981
Glucose and Cyclic AMP as Stimulators of Somatostatin and Insulin Secretion From the Isolated, Perfused Rat Pancreas: A Quantitative Study
Pietro P G Gerber;
Pietro P G Gerber
Institut de Biochimie Clinique, University of Geneva
Sentier de la Roseraie, 1211 Geneva 4, Switzerland
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Elisabeth R Trimble;
Elisabeth R Trimble
Institut de Biochimie Clinique, University of Geneva
Sentier de la Roseraie, 1211 Geneva 4, Switzerland
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Claes B Wollheim;
Claes B Wollheim
Institut de Biochimie Clinique, University of Geneva
Sentier de la Roseraie, 1211 Geneva 4, Switzerland
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Albert E Renold;
Albert E Renold
Institut de Biochimie Clinique, University of Geneva
Sentier de la Roseraie, 1211 Geneva 4, Switzerland
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Ralph E Miller
Ralph E Miller
Institut de Biochimie Clinique, University of Geneva
Sentier de la Roseraie, 1211 Geneva 4, Switzerland
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1
Dr. Miller's present address is Albert Chandler Medical Center, College of Medicine, Department of Pharmacology, University of Kentucky, Lexington, KY 40536.
Address reprint requests to Dr. Elisabeth Trimble, Institut de Biochimie Clinique, Sentier de la Roseraie, 1211 Geneva 4, Switzerland.
Diabetes 1981;30(1):40–44
Article history
Received:
May 01 1980
Revision Received:
August 12 1980
PubMed:
6164586
Citation
Pietro P G Gerber, Elisabeth R Trimble, Claes B Wollheim, Albert E Renold, Ralph E Miller; Glucose and Cyclic AMP as Stimulators of Somatostatin and Insulin Secretion From the Isolated, Perfused Rat Pancreas: A Quantitative Study. Diabetes 1 January 1981; 30 (1): 40–44. https://doi.org/10.2337/diab.30.1.40
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