Although the α- and β-adrenergic receptors have opposing effects on insulin secretion, the inhibitory influence of α-receptors appears to predominate. To determine if this was due to differences in number and affinity of receptors, isolated rat pancreatic islet cells were incubated with [3H]-dihydroalprenolol and [3H]-di-hydroergocryptine as ligands for β- and α-adrenergic binding sites. It was found that the number of β-adrenergic binding sites was 143 fmol/mg islet protein with a Kd = 0.57 nM. The number of α-adrenergic binding sites was 53 fmol/mg protein with a Kd = 0.26 nM. Thus, there are 2.7 times as many β-adrenergic binding sites as α-binding sites, and neither binding site number nor affinity is responsible for the predominant influence of the α-adrenergic receptors.
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February 01 1981
Preponderance of β-Adrenergic Binding Sites in Pancreatic Islet Cells of the Rat
Bruce Cherksey;
Bruce Cherksey
Department of Physiology and Biophysics, the Department of Ophthalmology, and the Department of Pharmacology, New York University Medical Center
550 First Avenue, New York, New York
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Norman Altszuler;
Norman Altszuler
Department of Physiology and Biophysics, the Department of Ophthalmology, and the Department of Pharmacology, New York University Medical Center
550 First Avenue, New York, New York
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Jose Zadunaisky
Jose Zadunaisky
Department of Physiology and Biophysics, the Department of Ophthalmology, and the Department of Pharmacology, New York University Medical Center
550 First Avenue, New York, New York
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Address reprint requests to Dr. Norman Altszuler, Department of Pharmacology, New York University Medical Center, 550 First Avenue, New York, New York 10016.
Citation
Bruce Cherksey, Norman Altszuler, Jose Zadunaisky; Preponderance of β-Adrenergic Binding Sites in Pancreatic Islet Cells of the Rat. Diabetes 1 February 1981; 30 (2): 172–174. https://doi.org/10.2337/diab.30.2.172
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