The present experiments indicate that ZnCl2 (0.015–0.50 mM) inhibits in a dose-dependent manner insulin secretion from isolated rat islets stimulated by D-glucose, L-leucine, and potassium. This inhibitory effect is partially reversed by washing and antagonized by high calcium concentrations in the medium. Zinc levels that inhibit insulin release do not affect 45calcium uptake, and zinc will not replace calcium in triggering insulin release. The conversion of 14C-D-glucose to 14CO2 by islets is not modified by zinc (0.12 mM or 0.50 mM) following either 2- or 0.5-h incubation periods, respectively. It is concluded that the inhibitory effect of zinc on insulin secretion may, in part, be mediated through interference with an intracellular function of calcium by the β-cell.
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Original contribution| April 01 1981
Zinc-Induced Inhibition of Insulin Secretion from Isolated Rat Islets of Langerhans
Michael L McDaniel;
Address reprint requests to Paul E. Lacy, Department of Pathology, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, Missouri 63110.
Taghi Ghafghazi, Michael L McDaniel, Paul E Lacy; Zinc-Induced Inhibition of Insulin Secretion from Isolated Rat Islets of Langerhans. Diabetes 1 April 1981; 30 (4): 341–345. https://doi.org/10.2337/diab.30.4.341
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