Monolayer cultures of neonatal rat pancreatic cells were examined to ascertain whether they synthesize prostaglandin E (PGE) and to determine the effects on insulin secretion caused by PGE and drugs that inhibit its synthesis. PGE release into the medium was observed. Sodium salicylate and ibuprofen (at drug concentrations similar to those achieved therapeutically in humans in vivo) inhibited PGE synthesis in a doseresponsive fashion to a maximum of 70–80% inhibition. Inhibition of PGE synthesis was accompanied by augmented insulin secretion. Both PGE synthesis inhibitors shifted the glucose dose-insulin response curves to the left at low glucose concentrations and augmented maximal insulin release at high glucose concentrations. Increments in glucose-induced insulin secretion induced by sodium salicylate correlated well (r = 0.89) with inhibition of PGE synthesis and addition of exogenous PGE1 to the cultures reversed the augmenting effects of the drug on insulin secretion. It is concluded that cultures of pancreatic cells synthesize PGE and that a function of PGE in these cultures appears to be a tonic negative modulation of glucose-induced insulin secretion.
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Original Contributions|
July 01 1981
Inhibition of Prostaglandin E Synthesis Augments Glucose-induced Insulin Secretion in Cultured Pancreas
Stewart A Metz;
Stewart A Metz
Divisions of Clinical Pharmacology, and Endocrinology and Metabolism, and the Department of Medicine, University of Washington; and the Seattle VA Medical Center
Seattle, Washington
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R Paul Robertson;
R Paul Robertson
Divisions of Clinical Pharmacology, and Endocrinology and Metabolism, and the Department of Medicine, University of Washington; and the Seattle VA Medical Center
Seattle, Washington
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Wilfred Y Fujimoto
Wilfred Y Fujimoto
Divisions of Clinical Pharmacology, and Endocrinology and Metabolism, and the Department of Medicine, University of Washington; and the Seattle VA Medical Center
Seattle, Washington
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Address reprint requests to Stewart A. Metz, Division of Clinical Pharmacology, Department of Medicine, Seattle VA Medical Center, 4435 Beacon Avenue South, Seattle, Washington 98108.
Diabetes 1981;30(7):551–557
Article history
Received:
June 26 1980
Revision Received:
March 03 1981
PubMed:
7018961
Citation
Stewart A Metz, R Paul Robertson, Wilfred Y Fujimoto; Inhibition of Prostaglandin E Synthesis Augments Glucose-induced Insulin Secretion in Cultured Pancreas. Diabetes 1 July 1981; 30 (7): 551–557. https://doi.org/10.2337/diab.30.7.551
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