Culture of isolated mouse pancreatic islets in RPMI 1640 for 4 wk with maintenance of specific functions is possible, although a considerable reduction of the number of surviving islets was observed. Culture in medium TC 199 resulted in a lower islet loss, but such islets were functionally less competent. Noncultured C57BL/6J (H-2b) islets were rapidly rejected when allografted intrasplenically into alloxan-diabetic but nonimmunosuppressed C57BL/KsJ (H-2d) mice. By contrast, the immunogenicity of C57BL/6J islets cultured for 4 wk at 37°C in RPM1 1640 and an air-carbon dioxide atmosphere was markedly reduced as evidenced by their ability to partly or completely normalize the hyperglycemia of nonimmunosuppressed alloxan-diabetic C57BL/KsJ mice for several weeks. Both intact islets and islets undergoing rejection were found in the spleens. The present data suggest that in vitro culture for 4 wk may reduce the immunogenicity of the islets, although a complete suppression is not achieved.
Skip Nav Destination
Article navigation
Reduction of Immunogenicity by Tissue Culture|
August 01 1982
Reversal of Hyperglycemia by Intrasplenic Transplantation of 4-Week-Cultured Allogeneic Mouse Islets
Arne Andersson
Arne Andersson
Department of Medical Cell Biology
P.O. Box 571, University of Uppsala, S-751 23 Uppsala, Sweden
Search for other works by this author on:
Address reprint requests to Arne Andersson M.D., at the above address
Citation
Arne Andersson; Reversal of Hyperglycemia by Intrasplenic Transplantation of 4-Week-Cultured Allogeneic Mouse Islets. Diabetes 1 August 1982; 31 (Supplement_4): 55–59. https://doi.org/10.2337/diab.31.4.S55
Download citation file: