The immunogenicity of purified pork insulins (PPI) with and without (groups 1 and 2,respectively) trace contamination of beef insulin was contrasted with mixed beef pork insulin of lower purity (MBP, group 3) in 137 patients who had not previously been treated with insulin. Patients and physicians were blinded with regard to the species source of insulin and studies were conductedfor a minimum of 1 yr. Antibody development to insulin was assessed by species-specific binding of 125I-insulin by acid charcoal extracted sera, as well as by measurement of insulin prebound to immunoglobulins by a polyethylene glycol precipitation method. NPH- and lente-treated individuals had equivalent antibody responses with regard to the rate of development of antibodies, and maximum immune responses to insulin. In all patient groups, antibody bound insulin as wellas species-specific binding of 125I-insulin increased significantly over time (all P < 0.01 for specific binding of pork and beef insulins SBP and SBB, as well as bound insulin). Maximum bound insulin and SBB as well as bound insulin and SBB over the entire course of the study were significantly greater in group 1 than in group 2 patients (both P < 0.05) The rate of development and magnitude of antibodies' responses in both PPI-treated groups were significantly less than that seen in the MBP group (all P < 0.01). New formation of antibeef proinsulin antibodies was seen in one patient from groups 1 and 3, but not in group 2. In all groups, insulin dose per day and fasting serum glucose concentrations increased by about 5 U/day and 10 mg/dl over 1 yr, but groups did not differ. MBP insulin used in these studies proved to be significantly less immunogenic than PPI alone.
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Original Articles|
July 01 1983
Effects of Species of Origin, Purification Levels, and Formulation on Insulin Immunogenicity
S Edwin Fineberg;
S Edwin Fineberg
Indiana University School of Medicine and Lilly Laboratories for Clinical Research
Indianapolis, Indiana
Endocrinology and Metabolism Laboratory, Henry Ford Hospital
Detroit, Michigan
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John A Galloway;
John A Galloway
Indiana University School of Medicine and Lilly Laboratories for Clinical Research
Indianapolis, Indiana
Endocrinology and Metabolism Laboratory, Henry Ford Hospital
Detroit, Michigan
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Naomi S Fineberg;
Naomi S Fineberg
Indiana University School of Medicine and Lilly Laboratories for Clinical Research
Indianapolis, Indiana
Endocrinology and Metabolism Laboratory, Henry Ford Hospital
Detroit, Michigan
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Jose Goldman
Jose Goldman
Indiana University School of Medicine and Lilly Laboratories for Clinical Research
Indianapolis, Indiana
Endocrinology and Metabolism Laboratory, Henry Ford Hospital
Detroit, Michigan
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Address reprint requests to S. Edwin Fineberg, M.D., Indiana University School of Medicine, Regenstrief Health Center, Room 603, 1001 West 10th Street, Indianapolis, Indiana 46202.
Diabetes 1983;32(7):592–599
Article history
Received:
August 17 1982
Revision Received:
January 04 1983
PubMed:
6345238
Citation
S Edwin Fineberg, John A Galloway, Naomi S Fineberg, Jose Goldman; Effects of Species of Origin, Purification Levels, and Formulation on Insulin Immunogenicity. Diabetes 1 July 1983; 32 (7): 592–599. https://doi.org/10.2337/diab.32.7.592
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