A technically simple radioimmunoassay for somatosta-tin-like immunoreactivity (SLI) in human plasma, which uses polyethylene glycol 6000 to precipitate tracer-degrading enzymes and high molecular weight interfering components, is described and validated. Fasting concentrations of SLI in peripheral plasma were similar in healthy and non-insulin-dependent diabetic (NIDD) subjects (range 3–39 pg/ml) but showed fluctuations for individuals in both groups when sampled frequently over a 60-min period. The mean coefficient of variation of SLI concentration was, however, less (8.0%; range 3–13%) in the diabetic group than in age-matched healthy subjects (19.5%; range 11–44%) (P < 0.02). Thus the regulation of basal D cell activity or of the somatostatinergic nervous system may be altered in type II diabetic subjects. A hospital breakfast (550 kcal) was a weak stimulus for release of SLI in both healthy and diabetic subjects. The rise in plasma SLI was sustained over 120 min. An increment in plasma SLI over mean basal levels of 5.9 ± 0.8 pg/ml was observed in young (mean age 23 yr; N = 6) control subjects, 7.9 ± 2.0 pg/ml in older (mean age 55 yr; N = 7) control subjects and 5.9 ± 0.6 pg/ml (mean age 62 yr; N = 7) in diabetics. Oral glucose (75 g) produced only a transient and erratic rise in plasma SLI that was not significant at any time point for either healthy or NIDD subjects. It is concluded that further investigations are necessary beforehypotheses linking somatostatin deficiency with the hormonal and metabolic abnormalities of non-insulin-dependent diabetes can be accepted.
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Original Articles|
August 01 1983
Circulating Somatostatin Concentrations in Healthy and Non-insulin-dependent (Type II) Diabetic Subjects
J M Conlon;
J M Conlon
Department of Clinical Biochemistry and Metabolic Medicine, Royal Victoria Infirmary, University of Newcastle upon Tyne
Newcastle upon Tyne NE1 4LP, England
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A J McCulloch;
A J McCulloch
Department of Clinical Biochemistry and Metabolic Medicine, Royal Victoria Infirmary, University of Newcastle upon Tyne
Newcastle upon Tyne NE1 4LP, England
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K G M M Alberti
K G M M Alberti
Department of Clinical Biochemistry and Metabolic Medicine, Royal Victoria Infirmary, University of Newcastle upon Tyne
Newcastle upon Tyne NE1 4LP, England
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Diabetes 1983;32(8):723–729
Article history
Received:
August 06 1982
Revision Received:
February 11 1983
PubMed:
6347769
Citation
J M Conlon, A J McCulloch, K G M M Alberti; Circulating Somatostatin Concentrations in Healthy and Non-insulin-dependent (Type II) Diabetic Subjects. Diabetes 1 August 1983; 32 (8): 723–729. https://doi.org/10.2337/diab.32.8.723
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