Proislets, derived from fetal mouse pancreata by collagenase digestion and subsequent organ culture, can be frozen to −196°C and stored in a viable condition before successful syngeneic transplantation.
Cryopreserved proislets are relatively undifferentiated morphologically, but continue to differentiate into mature islets after transplantation.
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Copyright © 1984 by the American Diabetes Association
1984
