In the present study, the effect of chloroquine on both insulin and receptor distribution was examined in vivo. Insulin injection (25 nmol/100 g body wt) caused a marked accumulation of both insulin and its receptor in purified hepatic Golgi fractions by 15 min postinjection. Percoll fractionation of parent Golgi fractions resolved two endocytic components of low (ρ = 1.040–1.050) and high (ρ = 1.053–1.064) density in which the relative distribution of insulin binding sites was unaltered by chloroquine. Chloroquine significantly accumulated in the high-density region of the Percoll gradient consistent with this being a low pH compartment. 125I-insulin accumulated first in the low-density (1 min) and subsequently in the high-density region (5–10 min) of Percollsubfractionated Golgi fractions. Chloroquine treatment caused marked accumulation of 125I-insulin in the highdensity compartment with substantial retention of radiolabel therein at 20 min postinjection. 125I-insulin extracted from the Percoll fractions was comparably intact in control and chloroquine-treated rats. These data suggest that the chloroquine-accumulating, high-density compartment of hepatic Golgi fractions is the site of dissociation of internalized insulin-receptor complexes before degradation of the ligand and receptor recycling.
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Original Contributions|
October 01 1985
Insulin and Insulin Receptor Uptake into Rat Liver: Chloroquine Action on Receptor Recycling
Masood N Khan;
Masood N Khan
Departments of Medicine and Anatomy. McGill University and the Royal Victoria Hospital
Montreal, Quebec, H3A 1A1, Canada
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Sylvine Savoie;
Sylvine Savoie
Departments of Medicine and Anatomy. McGill University and the Royal Victoria Hospital
Montreal, Quebec, H3A 1A1, Canada
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Rahat J Khan;
Rahat J Khan
Departments of Medicine and Anatomy. McGill University and the Royal Victoria Hospital
Montreal, Quebec, H3A 1A1, Canada
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John J M Bergeron;
John J M Bergeron
Departments of Medicine and Anatomy. McGill University and the Royal Victoria Hospital
Montreal, Quebec, H3A 1A1, Canada
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Barry I Posner
Barry I Posner
Departments of Medicine and Anatomy. McGill University and the Royal Victoria Hospital
Montreal, Quebec, H3A 1A1, Canada
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Address reprint requests to Barry I. Posner, M.D., Department of Medicine, Royal Victoria Hospital. Montreal, Quebec, H3A 1A1, Canada.
Diabetes 1985;34(10):1025–1030
Article history
Received:
September 08 1984
Revision Received:
March 13 1985
PubMed:
3899805
Citation
Masood N Khan, Sylvine Savoie, Rahat J Khan, John J M Bergeron, Barry I Posner; Insulin and Insulin Receptor Uptake into Rat Liver: Chloroquine Action on Receptor Recycling. Diabetes 1 October 1985; 34 (10): 1025–1030. https://doi.org/10.2337/diab.34.10.1025
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