The functional and morphologic characteristics of freedraining, pancreatic segmental autografts (FDPS) were studied in 8 beagle dogs that had survived longer than 4 yr. After pancreatectomy, animals received FDPS autografts of the left pancreatic limb, representing approximately one-third of the total pancreas with iliac vessel anastamoses. The grafted recipients were given pancreatic enzyme supplements (Viokase). After transplantation (tx), all 8 animals sustained fasting euglycemia with no evidence of microvascular complications. After 4 yr, IVGTT revealed K-values (%/min) that were not significantly different from age-matched controls (2.9 ± 0.5 versus 3.7 ± 0.6, P > 0.05). Mean fasting serum insulin levels were significantly greater in the tx animals (49 ± 5 μU/ml versus 12.2 μU/ml, P < 0.001), although the incremental response to i.v. glucose (0.5 g/kg) was less than in controls (P < 0.05). Mean fasting plasma C-peptide levels (0.09 ± 0.01 pmol/ml versus 0.21 ± 0.5 pmol/ml) and peak C-peptide responses to i.v. glucose were both significantly less than in controls. Sequential pancreatic biopsies up to 2.5 yr post-tx showed atrophy of the exocrine pancreas with coalescence of islets and mild fibrosis that did not progress with time. Immunoperoxidase stains confirmed the presence of insulin, glucagon, and somatostatin within nests of islet cells. Four years after transplantation of FDPS autografts in pancreatectomized dogs, excellent function is retained. The consequences of peripheral hyperinsulinemia remain to be determined.
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Original Contributions|
February 01 1985
Long-Term Follow-up of Canine Segmental Pancreatic Autografts
Richard G Cutfield;
Richard G Cutfield
Department of Medicine and Surgery, University of Miami School of Medicine
1600 Northwest 10th Avenue, Miami, Florida
Department of Medicine, University of Chicago, Pritzker School of Medicine
Chicago. Illinois
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Kenneth Polonsky;
Kenneth Polonsky
Department of Medicine and Surgery, University of Miami School of Medicine
1600 Northwest 10th Avenue, Miami, Florida
Department of Medicine, University of Chicago, Pritzker School of Medicine
Chicago. Illinois
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Les Olson;
Les Olson
Department of Medicine and Surgery, University of Miami School of Medicine
1600 Northwest 10th Avenue, Miami, Florida
Department of Medicine, University of Chicago, Pritzker School of Medicine
Chicago. Illinois
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George Kyriakides;
George Kyriakides
Department of Medicine and Surgery, University of Miami School of Medicine
1600 Northwest 10th Avenue, Miami, Florida
Department of Medicine, University of Chicago, Pritzker School of Medicine
Chicago. Illinois
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Joshua Miller;
Joshua Miller
Department of Medicine and Surgery, University of Miami School of Medicine
1600 Northwest 10th Avenue, Miami, Florida
Department of Medicine, University of Chicago, Pritzker School of Medicine
Chicago. Illinois
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Daniel H Mintz
Daniel H Mintz
Department of Medicine and Surgery, University of Miami School of Medicine
1600 Northwest 10th Avenue, Miami, Florida
Department of Medicine, University of Chicago, Pritzker School of Medicine
Chicago. Illinois
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Address reprint requests to Daniel H Mintz. M.D.. University of Miami School of Medicine, Department of Medicine, Division of Endocrinology, P. 0. Box 016960, Miami, Florida 33101.
Diabetes 1985;34(2):174–178
Article history
Received:
May 15 1984
Revision Received:
August 03 1984
PubMed:
2857144
Citation
Richard G Cutfield, Kenneth Polonsky, Les Olson, George Kyriakides, Joshua Miller, Daniel H Mintz; Long-Term Follow-up of Canine Segmental Pancreatic Autografts. Diabetes 1 February 1985; 34 (2): 174–178. https://doi.org/10.2337/diab.34.2.174
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