Islets from male B10.BR mice (H-2k) were isolated by the collagenase technique, handpicked with a Pasteur pipette, and incubated (either intact or after dispersion with Dispase) for 0, 3, 5, 7,10, or 14 days in tissue culture medium supplemented with either lymphokine supernatants or recombinant murine interferon-γ. Islets and single cells were examined for IAk molecules by use of indirect immunofluorescence. la-positive islet cells were identified on the surface of islets incubated with 5–10% lymphokine for >4 days or with 10,100, or 1000 ng/ml interferon for >6 days. Islets incubated in unsupplemented medium were la negative. Incubation with 5% lymphokine induced la expression on 10–40% dispersed islet cells cultured for >9 days. Dual immunofluorescent staining for la and insulin revealed that la-positive cells included both β- and non-β-cells.
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October 01 1986
Interferon-Mediated Induction of la Antigen Expression on Isolated Murine Whole Islets and Dispersed Islet Cells
J R Wright, Jr;
J R Wright, Jr
Departments of Pathology and Genetics, Washington University School of Medicine
St. Louis, Missouri
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P E Lacy;
P E Lacy
Departments of Pathology and Genetics, Washington University School of Medicine
St. Louis, Missouri
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E R Unanue;
E R Unanue
Departments of Pathology and Genetics, Washington University School of Medicine
St. Louis, Missouri
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C Muszynski;
C Muszynski
Departments of Pathology and Genetics, Washington University School of Medicine
St. Louis, Missouri
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V Hauptfeld
V Hauptfeld
Departments of Pathology and Genetics, Washington University School of Medicine
St. Louis, Missouri
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Address reprint requests to James R. Wright, Jr., MD, Department of Pathology, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110.
Diabetes 1986;35(10):1174–1177
Article history
Received:
June 09 1986
Revision Received:
July 17 1986
PubMed:
3093300
Citation
J R Wright, P E Lacy, E R Unanue, C Muszynski, V Hauptfeld; Interferon-Mediated Induction of la Antigen Expression on Isolated Murine Whole Islets and Dispersed Islet Cells. Diabetes 1 October 1986; 35 (10): 1174–1177. https://doi.org/10.2337/diab.35.10.1174
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