A method for mass isolation of islets from the pig pancreas is described. The procedure involves the use of an enzymatic and mechanical pancreatic digestion procedure followed by filtration and separation of the islets on Ficoll gradients. A remarkably high yield of purified islets has been obtained from the pig pancreas with this procedure. The islets are morphologically intact and respond to acute stimulation with glucose in Vitro.
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Original Contribution| June 01 1986
A Method for the Mass Isolation of Islets From the Adult Pig Pancreas
Edward H Finke;
Address reprint requests to Dr. P. E. Lacy, Department of Pathology, Washington University Medical Center, 660 S. Euclid Ave., St. Louis, Missouri 63110.
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Camillo Ricordi, Edward H Finke, Paul E Lacy; A Method for the Mass Isolation of Islets From the Adult Pig Pancreas. Diabetes 1 June 1986; 35 (6): 649–653. https://doi.org/10.2337/diab.35.6.649
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