Offspring of diabetic humans and laboratory animals have been shown to have a higher incidence of congenital malformations with attendant growth retardation. These defects have been attributed to alterations in the intrauterine environment and specifically to changes in maternal serum factors, e.g., glucose and ketone bodies. Our investigation examines the potential teratogenicity of a low-molecular-weight (940) serum fraction with demonstrated somatomedin inhibitory activity isolated by column chromatography from streptozocin-induced diabetic rats. Mouse embryos were exposed to control or the inhibitor fraction at concentrations of 0.25–0.6% vol/vol (0.9–3.0 μg protein/ml culture medium) in whole embryo culture and evaluated for the presence of malformations and growth retardation. Embryos exposed to inhibitor during the period of neurulation (3–5 somites) exhibited neural tube and craniofacial defects, whereas those exposed during early limb bud stages (18–19 somites) exhibited abnormalities of the forebrain and face. In addition, both stages were growth retarded. Control fractions produced no abnormalities. These results demonstrate a potential role for somatomedin inhibitors in diabetic embryopathy and suggest that factors other than hyperglycemie and hyperketonemia may contribute to the higher incidence of malformations among infants of diabetic mothers.
Skip Nav Destination
Article navigation
Original Contributions|
August 01 1986
Somatomedin Inhibitors From Diabetic Rat Serum Alter Growth and Development of Mouse Embryos in Culture
T W Sadler;
T W Sadler
Department of Anatomy, School of Medicine, University of North Carolina
Chapel Hill, North Carolina
Search for other works by this author on:
L S Phillips;
L S Phillips
Department of Medicine, Division of Endocrinology and Metabolism, School of Medicine, Emory University
Atlanta, Georgia
Search for other works by this author on:
W Balkan;
W Balkan
Department of Anatomy, School of Medicine, University of North Carolina
Chapel Hill, North Carolina
Search for other works by this author on:
S Goldstein
S Goldstein
Department of Medicine, Division of Endocrinology and Metabolism, School of Medicine, Emory University
Atlanta, Georgia
Search for other works by this author on:
Address reprint requests to T. W. Sadler, Department of Anatomy, 111 Swing Building, 217H, School of Medicine, University of North Carolina, Chapel Hill, NC 27514.
Diabetes 1986;35(8):861–865
Article history
Received:
December 07 1985
Revision Received:
March 03 1986
PubMed:
3732629
Citation
T W Sadler, L S Phillips, W Balkan, S Goldstein; Somatomedin Inhibitors From Diabetic Rat Serum Alter Growth and Development of Mouse Embryos in Culture. Diabetes 1 August 1986; 35 (8): 861–865. https://doi.org/10.2337/diab.35.8.861
Download citation file:
46
Views