The accumulation of polyols has been previously found in renal glomeruli isolated from streptozocin-induced diabetic (STZ-D) rats, although the intraglomerular polyol pathway has not been exactly localized. Because we have previously observed mesangial cell dysfunction in STZ-D rats, we examined whether the polyol pathway exists in mesangial cells as a possible candidate of the cause of cellular dysfunction. The activities of two polyol pathway enzymes, aldose reductase and sorbitol dehydrogenase, were clearly detected in the crude homogenate of cultured mesangial cells at higher levels than those of whole glomeruli when DL-glyceraldehyde or D-fructose was used as substrate. When cells were incubated in medium containing 55 mM glucose or galactose, a large amount of sorbitol or galactitol was accumulated intracellularly. The accumulation of polyolswas effectively blocked by an aldose reductase inhibitor, ICI 128436. These results suggest that the polyol pathway exists in mesangial cells of rat glomeruli and may play a role in the development of mesangial cell dysfunction found in STZ-D rats.
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February 01 1987
Evidence for Existence of Polyol Pathway in Cultured Rat Mesangial Cells
Ryuichi Kikkawa;
Ryuichi Kikkawa
Third Department of Medicine, Shiga University of Medical Science
Otsu, Shiga, Japan
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Kisaburo Umemura;
Kisaburo Umemura
Third Department of Medicine, Shiga University of Medical Science
Otsu, Shiga, Japan
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Masakazu Haneda;
Masakazu Haneda
Third Department of Medicine, Shiga University of Medical Science
Otsu, Shiga, Japan
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Tetsuro Arimura;
Tetsuro Arimura
Third Department of Medicine, Shiga University of Medical Science
Otsu, Shiga, Japan
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Kazuhiko Ebata;
Kazuhiko Ebata
Third Department of Medicine, Shiga University of Medical Science
Otsu, Shiga, Japan
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Yukio Shigeta
Yukio Shigeta
Third Department of Medicine, Shiga University of Medical Science
Otsu, Shiga, Japan
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Address reprint requests to Dr. Ryuichi Kikkawa, Third Department of Medicine, Shiga University of Medical Science, Seta, Otsu, Shiga, 520-21, Japan.
Diabetes 1987;36(2):240–243
Article history
Received:
September 22 1986
Revision Received:
October 23 1986
Accepted:
October 23 1986
PubMed:
3100369
Citation
Ryuichi Kikkawa, Kisaburo Umemura, Masakazu Haneda, Tetsuro Arimura, Kazuhiko Ebata, Yukio Shigeta; Evidence for Existence of Polyol Pathway in Cultured Rat Mesangial Cells. Diabetes 1 February 1987; 36 (2): 240–243. https://doi.org/10.2337/diab.36.2.240
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