Euglycemic-hyperinsulinemic clamps were performed on 4- and 12-wk-old anesthetized lean and obese Zucker rats. During the clamp studies, total glucose production and utilization were assessed with a 3-[3H]glucose perfusion, whereas local glucose utilization was determined by measuring 2-deoxy-1-[3H]glucose 6-phosphate accumulation in various tissues. In the basal state, 4 wk-old obese rats were hyperinsulinemic (159 ± 8 vs. 82 ± 9 μU/ml), whereas glucose turnover rate was similar to that observed in lean rats (14.9 ± 1.9 vs. 12.5 ± 1.9 mg · min−1 · kg−1). Glucose utilization was identical in skeletal muscles, whereas it was increased in white adipose tissue of obese rats (22 ± 4 vs. 8 ± 2 ng · min−1 · mg−1). At plasma insulin level of 500 μU/ml, glucose production was totally suppressed in both groups, whereas overall glucose utilization was slightly less in 4-wk-old obese than in lean rats. This was due to a reduced stimulation of glucose utilization in skeletal muscles and brown adipose tissue. In contrast, glucose utilization in periovarian white adipose tissue was similarly increased in lean and obese rats. For a maximal insulin concentration (1500 μU/ml), all the differences were abolished between lean and obese young Zucker rats. In older (12-wk-old) obese rats, glucose utilization in various tissues was markedly reduced at maximal insulin level compared with that observed in age-matched lean animals. Thus, a decreased insulin sensitivity in skeletal muscles and brown adipose tissue with a normal insulin sensitivity in white adipose tissue could contribute to the development of obesity in young Zucker rats by preferentially channeling glucose toward this tissue.
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May 01 1987
Development of Obesity in Zucker Rats: Early Insulin Resistance in Muscles But Normal Sensitivity in White Adipose Tissue
Luc Pénicaud;
Luc Pénicaud
Laboratoire de Physiologie du Développement, Université Paris VII
LA 307 du CNRS, Paris, France
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Pascal Ferré;
Pascal Ferré
Centre de Recherches sur La Nutrition du CNRS
Meudon Bellevue, France
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Jacques Terretaz;
Jacques Terretaz
Laboratoires de Recherches Métaboliques
Geneva, Switzerland
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Marie France Kinebanyan;
Marie France Kinebanyan
Laboratoire de Physiologie du Développement, Université Paris VII
LA 307 du CNRS, Paris, France
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Armelle Leturque;
Armelle Leturque
Centre de Recherches sur La Nutrition du CNRS
Meudon Bellevue, France
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Elizabeth Doré;
Elizabeth Doré
Laboratoire de Physiologie du Développement, Université Paris VII
LA 307 du CNRS, Paris, France
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Jean Girard;
Jean Girard
Centre de Recherches sur La Nutrition du CNRS
Meudon Bellevue, France
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Bernard Jeanrenaud;
Bernard Jeanrenaud
Laboratoires de Recherches Métaboliques
Geneva, Switzerland
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Luc Picon
Luc Picon
Laboratoire de Physiologie du Développement, Université Paris VII
LA 307 du CNRS, Paris, France
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Address correspondence and reprint requests to Dr. L. Pénicaud, Laboratoire de Physiologie du Développement, Université Paris 7, Tour 23-33, 1er étage, 2 Place Jussieu, 75251 Paris Cedex 05, France.
Diabetes 1987;36(5):626–631
Article history
Received:
July 28 1986
Revision Received:
November 13 1986
Accepted:
November 13 1986
PubMed:
3552794
Citation
Luc Pénicaud, Pascal Ferré, Jacques Terretaz, Marie France Kinebanyan, Armelle Leturque, Elizabeth Doré, Jean Girard, Bernard Jeanrenaud, Luc Picon; Development of Obesity in Zucker Rats: Early Insulin Resistance in Muscles But Normal Sensitivity in White Adipose Tissue. Diabetes 1 May 1987; 36 (5): 626–631. https://doi.org/10.2337/diab.36.5.626
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