Proliferation of vascular smooth muscle is thought to be involved in the major diabetic complication atherosclerosis. We have previously reported an increase of growth-promoting activity (GA) in platelets from insulin-dependent diabetics. In this study, GA was measured in the platelet extract (PE) from eight diabetic patients who had been treated by conventional insulin therapy. Vascular smooth muscle cells from rat aorta were cultured and used as an assay system for GA. Incorporation of [3H]thymidine into DNA of cultured cells was stimulated by diabetic PE significantly more (P < .05) than by normal PE. Diabetic PE incubated with cells for 4 days increased cell numbers significantly more (P < .05) than normal PE. These abnormalities were corrected by long-term intensive insulin treatments (continuous subcutaneous insulin infusion and Pen infuser). The decrease of platelet extract GA appeared to correlate with the amount of insulin administered before meals as shortacting boluses, whereas the level of basal or longacting insulin appeared to correlate with an increase of PE GA. Thus, the growth-promoting potential of platelets can be normalized by intensive insulin therapy. The relationship of insulin levels to this activity needs further evaluation.
Skip Nav Destination
Article navigation
Original Contributions|
May 01 1987
Therapeutic Modulation of Growth-Promoting Activity in Platelets From Diabetics
Hidekatsu Sugimoto;
Hidekatsu Sugimoto
Clinical Research Institute of Montreal
Montreal, Quebec, Canada
Search for other works by this author on:
Douglas J Franks;
Douglas J Franks
Clinical Research Institute of Montreal
Montreal, Quebec, Canada
Search for other works by this author on:
Louise Lecavalier;
Louise Lecavalier
Clinical Research Institute of Montreal
Montreal, Quebec, Canada
Search for other works by this author on:
Jean-Louis Chiasson;
Jean-Louis Chiasson
Clinical Research Institute of Montreal
Montreal, Quebec, Canada
Search for other works by this author on:
Pavel Hamet
Pavel Hamet
Clinical Research Institute of Montreal
Montreal, Quebec, Canada
Search for other works by this author on:
Address correspondence and reprint requests to Dr. P. Harriet, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, H2W 1R7, Canada.
Diabetes 1987;36(5):667–672
Article history
Received:
June 24 1986
Revision Received:
December 17 1986
Accepted:
December 17 1986
PubMed:
3552800
Citation
Hidekatsu Sugimoto, Douglas J Franks, Louise Lecavalier, Jean-Louis Chiasson, Pavel Hamet; Therapeutic Modulation of Growth-Promoting Activity in Platelets From Diabetics. Diabetes 1 May 1987; 36 (5): 667–672. https://doi.org/10.2337/diab.36.5.667
Download citation file:
33
Views