We examined the ability of fibronectin, an extracellular glycoprotein that interacts with cell surfaces and matrix components, to bind to glomerular basement membrane and the effect of diabetes on this binding. 125I-Iabeled fibronectin binding to rat glomerular basement membrane (GBM) was dose dependent, related to time and amount of basement membrane, and inhibited by unlabeled fibronectin but not by unrelated proteins. Binding was reduced ∼60% when GBM was pretreated with collagenase and ∼24% when pretreated with chondroitinase plus heparinase. Treatment with NaCI had little effect on binding, whereas reduction with p-mercaptoethanol removed ∼25% of the bound 125I-fibronectin. Binding to samples prepared from rats with streptozocin-induced diabetes was significantly increased compared with that observed with control preparations at all concentrations of fibronectin and of basement membrane tested. The findings provide direct evidence that fibronectin binds to GBM and that this binding, which represents a biologic function of the protein, is enhanced in diabetes.
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Original Contributions|
June 01 1987
Fibronectin Binding to Glomerular Basement Membrane is Altered in Diabetes
Margo Panush Cohen;
Margo Panush Cohen
University of Medicine and Dentistry of New Jersey
Newark, New Jersey
, and the University City Science Center
Philadelphia, Pennsylvania
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Romesh Saini;
Romesh Saini
University of Medicine and Dentistry of New Jersey
Newark, New Jersey
, and the University City Science Center
Philadelphia, Pennsylvania
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Henry Klepser;
Henry Klepser
University of Medicine and Dentistry of New Jersey
Newark, New Jersey
, and the University City Science Center
Philadelphia, Pennsylvania
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L G Vasanthi
L G Vasanthi
University of Medicine and Dentistry of New Jersey
Newark, New Jersey
, and the University City Science Center
Philadelphia, Pennsylvania
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Address correspondence and reprint requests to Dr. Margo P. Cohen, Institute for Metabolic Research, University City Science Center, 3508 Market Street, Suite 420, Philadelphia, PA 19104.
Diabetes 1987;36(6):758–763
Article history
Received:
March 12 1986
Revision Received:
December 01 1986
Accepted:
December 01 1986
PubMed:
3569674
Citation
Margo Panush Cohen, Romesh Saini, Henry Klepser, L G Vasanthi; Fibronectin Binding to Glomerular Basement Membrane is Altered in Diabetes. Diabetes 1 June 1987; 36 (6): 758–763. https://doi.org/10.2337/diab.36.6.758
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