Our attempt to reduce islet immunogenicity by slow cooling to −40°C, storage at −196°C, and rapid thawing is based on the differential susceptibility of various cell types to a freeze-thaw process. Five hundred rat islets (≥10 μm) were immediately implanted or cryopreserved and then implanted beneath the renal capsule of streptozocin-induced diabetic mice with or without an injection of anti-lymphocyte serum at the time of transplantation. Thirteen days after transplantation, all fresh xenografts had rejected, whereas 37.5% of cryopreserved grafts were still functioning. In immunosuppressed mice, 6.2% of fresh xenografts and 54.5% of cryopreserved grafts were functioning 19 days after transplantation. These results show that cryopreservation can extend xenograft survival.
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Rapid Publication| September 01 1987
Prolongation of Islet Xenograft Survival by Cryopreservation
Marilyne G Coulombe;
Garth L Warnock;
Address correspondence and reprint requests to Dr. R. V. Rajotte, Surgical Medical Research Institute, 1074A Dentistry Pharmacy Building, University of Alberta, Edmonton, Alberta, Canada T6G 2N8.
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Marilyne G Coulombe, Garth L Warnock, Ray V Rajotte; Prolongation of Islet Xenograft Survival by Cryopreservation. Diabetes 1 September 1987; 36 (9): 1086–1088. https://doi.org/10.2337/diab.36.9.1086
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