In this study we examined the effect of experimental diabetes and of treatment with an aldose reductase inhibitor on the level of sulfation of glomerular basement membrane (GBM) heparan sulfate, the principal glycosaminoglycan in this extracellular matrix. Glycosaminoglycans were isolated from GBM purified from control, streptozocin-induced diabetic, and sorbinil-treated diabetic rats and analyzed for sulfate and uronate content. Glomerular yields from diabetic kidneys were greater than those from control animals, but the amount of sulfate per glomerulus in diabetic samples, both untreated and sorbinil treated, did not differ significantly from that in control samples. However, the sulfate-to-uronate ratio in heparan sulfate isolated from diabetic GBM (0.34 ± 0.08) was significantly less than in control samples (0.69 ± 0.11), and treatment of diabetic rats with an aldose reductase inhibitor did not correct this reduced ratio (0.36 ± 0.06). The results indicate that there is an undersulfation of heparan sulfate of GBM in experimental diabetes, an abnormality that may contribute to loss of anionie sites and decreased charge selectivity of the glomerular filtration barrier. The findings further suggest that this abnormality results from disturbances in glycosaminoglycan synthesis and/or metabolism in diabetes that are independent of polyol-pathway activation in the renal glomerulus.
Skip Nav Destination
Article navigation
Original Articles|
October 01 1988
Undersulfation of Glomerular Basement Membrane Heparan Sulfate in Experimental Diabetes and Lack of Correction With Aldose Reductase Inhibition
Margo Panush Cohen;
Margo Panush Cohen
University City Science Center
Philadelphia, Pennsylvania
Search for other works by this author on:
Henry Klepser;
Henry Klepser
University City Science Center
Philadelphia, Pennsylvania
Search for other works by this author on:
Van-Yu Wu
Van-Yu Wu
University City Science Center
Philadelphia, Pennsylvania
Search for other works by this author on:
Address correspondence and reprint requests to Margo P. Cohen, MD, University City Science Center, 3508 Market Street, Suite 420, Philadelphia, PA 19104.
Diabetes 1988;37(10):1324–1327
Article history
Received:
November 13 1987
Revision Received:
May 05 1988
Accepted:
May 05 1988
PubMed:
2970980
Citation
Margo Panush Cohen, Henry Klepser, Van-Yu Wu; Undersulfation of Glomerular Basement Membrane Heparan Sulfate in Experimental Diabetes and Lack of Correction With Aldose Reductase Inhibition. Diabetes 1 October 1988; 37 (10): 1324–1327. https://doi.org/10.2337/diab.37.10.1324
Download citation file:
37
Views