ATPase activities were measured in sciatic nerves from rats with alloxan-induced diabetes (ALX-D) of various duration (2 wk, 5 wk, 9 wk, and 6 mo). Our data confirm that sciatic nerve Na+-K+-ATPase abnormalities are present very early in ALX-D rats, similar to results previously described in streptozocin-induced diabetic rats, spontaneously diabetic BB Wistar rats, and ALX-D rabbits. Na+-K+-ATPase activity decreased by 26–47% in ALX-D rats compared with age-matched controls. Ganglioside treatment (10 mg/kg i.p. for 10 or 30 days starting 1 wk after ALX injection) completely impeded the enzyme reduction. The effect observed at the end of either 10 or 30 days of treatment lasted ≥1 mo. Chronic diabetic groups treated for 30 days before killing also presented normal ATPase activity at the end of treatment. Therefore, gangliosides are effective on Na+-K+-ATPase even in animals with a longer duration of diabetes. The maintenance of fairly normal ATPase activity by ganglioside treatment could mirror a more general recovery from early metabolic dysfunction and/or late structural abnormalities in diabetic nerve fibers.
Skip Nav Destination
Article navigation
Original Articles|
October 01 1988
ATPase Activity Defects in Alloxan-Induced Diabetic Sciatic Nerve Recovered by Ganglioside Treatment
Roberto Bianchi;
Roberto Bianchi
“Mario Negri” Institute tor Pharmacological Research
Milan, and Fidia Research Laboratories, Abano Terme (PD), Italy
Search for other works by this author on:
Paolo Marini;
Paolo Marini
“Mario Negri” Institute tor Pharmacological Research
Milan, and Fidia Research Laboratories, Abano Terme (PD), Italy
Search for other works by this author on:
Serena Merlini;
Serena Merlini
“Mario Negri” Institute tor Pharmacological Research
Milan, and Fidia Research Laboratories, Abano Terme (PD), Italy
Search for other works by this author on:
Michele Fabris;
Michele Fabris
“Mario Negri” Institute tor Pharmacological Research
Milan, and Fidia Research Laboratories, Abano Terme (PD), Italy
Search for other works by this author on:
Chiara Triban;
Chiara Triban
“Mario Negri” Institute tor Pharmacological Research
Milan, and Fidia Research Laboratories, Abano Terme (PD), Italy
Search for other works by this author on:
Emilio Mussini;
Emilio Mussini
“Mario Negri” Institute tor Pharmacological Research
Milan, and Fidia Research Laboratories, Abano Terme (PD), Italy
Search for other works by this author on:
Mario G Fiori
Mario G Fiori
“Mario Negri” Institute tor Pharmacological Research
Milan, and Fidia Research Laboratories, Abano Terme (PD), Italy
Search for other works by this author on:
Address correspondence and reprint requests to Dr. Roberto Bianchi, Istituto di Ricerche Farmacologiche “Mario Negri,” Via Eritrea 62, 20157 Milan, Italy.
Diabetes 1988;37(10):1340–1345
Article history
Received:
January 04 1988
Revision Received:
May 05 1988
Accepted:
May 05 1988
PubMed:
2843406
Citation
Roberto Bianchi, Paolo Marini, Serena Merlini, Michele Fabris, Chiara Triban, Emilio Mussini, Mario G Fiori; ATPase Activity Defects in Alloxan-Induced Diabetic Sciatic Nerve Recovered by Ganglioside Treatment. Diabetes 1 October 1988; 37 (10): 1340–1345. https://doi.org/10.2337/diab.37.10.1340
Download citation file:
39
Views