High-performance liquid chromatography analysis of acid-extracted tissues revealed decreases of high-energy nucleotides and increases in low-energy nucleotides and metabolites in heart, diaphragm, and liver but not in kidneys of diabetic rats. In comparison with nondiabetic rats, the total adenine nucleotide content of diabetic rat heart and diaphragm but not liver decreased, indicating an increase in catabolism of AMP. Maximal initial rates of the AMP catabolic enzymes 5′-nucleotidase, adenosine deaminase, and AMP deaminase were elevated in the hearts of BB/Wistar and streptozocin-induced diabetic rats. Nucleotide salvage enzymes adenylosuccinate synthetase and adenylosuccinate lyase were elevated above normal in the diabetic heart, whereas hypoxanthine-guanine phosphoribosyl transferase was not altered. Cytosolic-to-mitochondrial ratios from maximal initial rates after correction for mitochondrial breakage were increased above controls in diabetic hearts for nucleoside diphosphokinase and aspartate aminotransferase. Nucleotide levels, degradation rates, and substrate compartmentation between cytosol and mitochondria are discussed in relation to concurrent diabetes.
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Original Articles|
May 01 1988
Adenine Nucleotide Metabolism in Hearts of Diabetic Rats: Comparison to Diaphragm, Liver, and Kidney
Ronald L Jenkins;
Ronald L Jenkins
Departments of Medicine and Surgery, University of Alabama at Birmingham, and the Veterans Administration Medical Center
Birmingham, Alabama
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Huey G McDaniel;
Huey G McDaniel
Departments of Medicine and Surgery, University of Alabama at Birmingham, and the Veterans Administration Medical Center
Birmingham, Alabama
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Stanley Digerness;
Stanley Digerness
Departments of Medicine and Surgery, University of Alabama at Birmingham, and the Veterans Administration Medical Center
Birmingham, Alabama
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S Wayne Parrish;
S Wayne Parrish
Departments of Medicine and Surgery, University of Alabama at Birmingham, and the Veterans Administration Medical Center
Birmingham, Alabama
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Richard L Ong
Richard L Ong
Departments of Medicine and Surgery, University of Alabama at Birmingham, and the Veterans Administration Medical Center
Birmingham, Alabama
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Address correspondence and reprint requests to Diabetes Research (151-G), VAMC, 700, 19th Street South, Birmingham, AL 35233.
Diabetes 1988;37(5):629–636
Article history
Received:
December 30 1986
Revision Received:
October 08 1987
Accepted:
October 08 1987
PubMed:
3360219
Citation
Ronald L Jenkins, Huey G McDaniel, Stanley Digerness, S Wayne Parrish, Richard L Ong; Adenine Nucleotide Metabolism in Hearts of Diabetic Rats: Comparison to Diaphragm, Liver, and Kidney. Diabetes 1 May 1988; 37 (5): 629–636. https://doi.org/10.2337/diab.37.5.629
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