Spontaneous diabetes was fully prevented in 65 BB/hooded (BB/h) highly diabetes-prone hybrid rats that were given five intraperitoneal injections (25 to 30 × 106 cells/injection) of fresh splenocytes or concanavalin A (ConA)–activated cultured splenocytes (blasts) from the diabetes-free Wistar-Furth or Long-Evans strains during the first 2 postnatal wk. Rats remained under observation for up to the age of 180–200 days. Of 70 littermate controls that received no cell injections, 63 developed overt diabetes before the age of 180 days. One intraperitoneal injection (25 × 106 cells) of splenocytes or blasts given during the first 36 h after birth was not as effective as multiple injections in preventing overt diabetes. Mild insulitis was present in 4 of 59 “protected” rats; small, discrete mononuclear infiltrates in periductular connective tissue and/or between pancreatic acini were observed in 27. Nondiabetic BB/h rats that were protected with splenocytes or blasts from diabetes-free strains had the same degree of lymphopenia in peripheral blood and spleen as age-matched, insulin-treated diabetic BB/h rats, but the level of islet cell surface antibodies in their serum was significantly lower. The same neonatal injections that protected rats from the development of spontaneous diabetes were completely ineffective in preventing the adoptive transfer of diabetes later in life by the injection of blasts from acutely diabetic BB/h rats.
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Original Articles|
August 01 1988
Prevention of Spontaneous But Not of Adoptively Transferred Diabetes by Injection of Neonatal BB/Hooded Hybrid Rats With Splenocytes or Concanavalin A Blasts From Diabetes-Free Strains
John Logothetopoulos;
John Logothetopoulos
Banting and Best Department of Medical Research, Charles H. Best Institute, and Department of Medicine and Pathology, University of Toronto
Toronto, Ontario, Canada
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Kenneth Shumak;
Kenneth Shumak
Banting and Best Department of Medical Research, Charles H. Best Institute, and Department of Medicine and Pathology, University of Toronto
Toronto, Ontario, Canada
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Dennis Bailey
Dennis Bailey
Banting and Best Department of Medical Research, Charles H. Best Institute, and Department of Medicine and Pathology, University of Toronto
Toronto, Ontario, Canada
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Address correspondence and reprint requests to Dr. John Logothetopoulos, Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6.
Diabetes 1988;37(8):1009–1014
Article history
Received:
September 28 1987
Revision Received:
February 10 1988
Accepted:
February 10 1988
PubMed:
3391342
Citation
John Logothetopoulos, Kenneth Shumak, Dennis Bailey; Prevention of Spontaneous But Not of Adoptively Transferred Diabetes by Injection of Neonatal BB/Hooded Hybrid Rats With Splenocytes or Concanavalin A Blasts From Diabetes-Free Strains. Diabetes 1 August 1988; 37 (8): 1009–1014. https://doi.org/10.2337/diab.37.8.1009
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