Diabetes was induced with streptozocin in male Wistar rats. After 4–6 mo, parotid salivary flow was induced by stimulating bipolar electrodes (3–5 V for 2 ms) on either the auriculotemporal nerve (5 Hz) or the cervical sympathetic trunk (10 Hz). During parasympathetic stimulation, flow rates were 443 ± 287 μl/30 min in diabetic animals compared to 657 ± 134 μl/30 min in controls (P < .01). Although parotid flow rates during parasympathetic stimulation were well maintained in controls, they decreased rapidly in most diabetic rats. Parotid secretory response to sympathetic stimulation was also reduced in diabetic compared with control animals (40 ± 16 μl/30 min and 82 ± 50 μl/30 min, respectively, P < .01). In contrast to results obtained with direct neural stimulation, no differences in threshold dose or total saliva were observed when cholinergic (methacholine) and peptidergic (physalaemin) agonists were administered intravenously. Response threshold for an adrenergic agonist (epinephrine) was significantly increased in diabetes. Electron microscopy showed axoplasmic abnormalities in nonmyelinated axons in diabetic glands but not in control glands. Although most nerve profiles appeared normal, neuroaxonal abnormalities were found in every diabetic animal. The ultrastructural findings and the similar responses to methacholine and physalaemin suggest that autonomie neuropathy may be a factor in the abnormal response of the parotid gland to parasympathetic nerve stimulation.
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Original Articles|
November 01 1989
Effects of Streptozocin-Induced Diabetes on Sympathetic and Parasympathetic Stimulation of Parotid Salivary Gland Function in Rats
Leigh C Anderson;
Leigh C Anderson
Department of Oral Biology, University of Washington
Seattle, Washington
Department of Oral Pathology, King's College Hospital School of Dentistry
London, United Kingdom
Department of Surgery, Jönköp-ing Lans Landsting
Jönköping, Sweden
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John R Garrett;
John R Garrett
Department of Oral Biology, University of Washington
Seattle, Washington
Department of Oral Pathology, King's College Hospital School of Dentistry
London, United Kingdom
Department of Surgery, Jönköp-ing Lans Landsting
Jönköping, Sweden
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Anders Thulin;
Anders Thulin
Department of Oral Biology, University of Washington
Seattle, Washington
Department of Oral Pathology, King's College Hospital School of Dentistry
London, United Kingdom
Department of Surgery, Jönköp-ing Lans Landsting
Jönköping, Sweden
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Gordon B Proctor
Gordon B Proctor
Department of Oral Biology, University of Washington
Seattle, Washington
Department of Oral Pathology, King's College Hospital School of Dentistry
London, United Kingdom
Department of Surgery, Jönköp-ing Lans Landsting
Jönköping, Sweden
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Address correspondence and reprint requests to Dr. Leigh C. Anderson, Department of Oral Biology, School of Dentistry, University of Washington, SB-22, Seattle, WA 98195.
Diabetes 1989;38(11):1381–1389
Article history
Received:
December 19 1988
Revision Received:
June 26 1989
Accepted:
June 26 1989
PubMed:
2482817
Citation
Leigh C Anderson, John R Garrett, Anders Thulin, Gordon B Proctor; Effects of Streptozocin-Induced Diabetes on Sympathetic and Parasympathetic Stimulation of Parotid Salivary Gland Function in Rats. Diabetes 1 November 1989; 38 (11): 1381–1389. https://doi.org/10.2337/diab.38.11.1381
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