Erythrocytes of diabetic patients have abnormal membrane properties. We examined in vivo membrane lipid peroxidation in erythrocytes of diabetic subjects and its possible relationship with hyperglycemia. Lipid peroxidation was assessed in fresh, untreated erythrocytes by quantitating thiobarbituric acid reactivity and an adduct of phospholipids and malonyldialdehyde (MDA), an end product of lipid peroxidation, with thin-layer chromatography of lipid extract of diabetic erythrocytes. There was a significantly increased membrane lipid peroxidation in diabetic erythrocytes compared with nondiabetic erythrocytes. The degree of membrane lipid peroxidative damage in erythrocytes was significantly correlated with the level of glycosylated hemoglobin, an index of mean glucose level for the preceding 3–4mo. This suggests that peroxidation of membrane lipids and accumulation of MDA occurs in erythrocytes of diabetic patients.
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Original Articles|
December 01 1989
Erythrocyte Membrane Lipid Peroxidation and Glycosylated Hemoglobin in Diabetes
Sushil K Jain;
Sushil K Jain
Department of Pediatrics, Louisiana State University School of Medicine
Shreveport, Louisiana
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Robert McVie;
Robert McVie
Department of Pediatrics, Louisiana State University School of Medicine
Shreveport, Louisiana
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John Duett;
John Duett
Department of Pediatrics, Louisiana State University School of Medicine
Shreveport, Louisiana
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John J Herbst
John J Herbst
Department of Pediatrics, Louisiana State University School of Medicine
Shreveport, Louisiana
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Address correspondence and reprint requests to Dr. Sushil K. Jain, Department of Pediatrics, Louisiana State University Medical Center, 1501 Kings Highway, Shreveport, LA 71130.
Diabetes 1989;38(12):1539–1543
Article history
Received:
March 20 1989
Revision Received:
July 19 1989
Accepted:
July 19 1989
PubMed:
2583378
Citation
Sushil K Jain, Robert McVie, John Duett, John J Herbst; Erythrocyte Membrane Lipid Peroxidation and Glycosylated Hemoglobin in Diabetes. Diabetes 1 December 1989; 38 (12): 1539–1543. https://doi.org/10.2337/diab.38.12.1539
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