Experiments were designed to gain information on the mechanisms leading to diabetic urinary bladder dysfunction. Bladders from control rats, animals subjected to 4–5 wk streptozocin-induced diabetes, and rats subjected to equivalent diuresis produced by 5% sucrose feeding were studied with an in vitro whole-bladder preparation and neurochemical measurements. The diuretic group was used to distinguish alterations produced by metabolic effects on nerve and muscle from those induced by prolonged periods of excessive diuresis. Diuresis alone explains many of the diabetes-induced effects, including decreased norepinephrine levels, postsynaptic supersensitivity for sympathetic regulation of bladder storage, decreased responsiveness to parasympathetic regulation of emptying, and enhanced prostaglandin F2α-induced contraction. Other diabetes-induced effects were not observed in the diuretic controls and are presumed to result from metabolic alterations associated with diabetes. These effects were decreases in norepinephrine uptake and in choline acetyltransferase activity, both markers of nerve terminal function. Thus, diuretic and metabolic factors appear to contribute to the early signs of parasympathetic and sympathetic neuropathy. In contrast, we found no evidence for loss of sensory nerve function in the diabetic bladder, at least at the organ level, because no diabetes- or diuresis-induced changes were observed in responsiveness to substance P or capsaicin.
Skip Nav Destination
Article navigation
Original Articles|
March 01 1989
Effects of Diabetes and Diuresis on Contraction and Relaxation Mechanisms in Rat Urinary Bladder
Elizabeth M Kudlacz;
Elizabeth M Kudlacz
Division of Pharmacology, College of Pharmacy, The Ohio State University
Columbus, Ohio
Search for other works by this author on:
Michael C Gerald;
Michael C Gerald
Division of Pharmacology, College of Pharmacy, The Ohio State University
Columbus, Ohio
Search for other works by this author on:
Lane J Wallace
Lane J Wallace
Division of Pharmacology, College of Pharmacy, The Ohio State University
Columbus, Ohio
Search for other works by this author on:
Address correspondence and reprint requests to Lane J. Wallace, College of Pharmacy, The Ohio State University, 500 West 12th Avenue, Columbus, OH 43210.
Diabetes 1989;38(3):278–284
Article history
Received:
March 02 1988
Revision Received:
September 07 1988
Accepted:
September 07 1988
PubMed:
2563711
Citation
Elizabeth M Kudlacz, Michael C Gerald, Lane J Wallace; Effects of Diabetes and Diuresis on Contraction and Relaxation Mechanisms in Rat Urinary Bladder. Diabetes 1 March 1989; 38 (3): 278–284. https://doi.org/10.2337/diab.38.3.278
Download citation file:
83
Views