The insulin release from isolated pancreatic islets grafted under the kidney capsule was examined by means of a modified kidney-perfusion technique. The grafts, consisting of 150 C57BL/6 or 250 C57BL/Ks mouse islets, were implanted syngeneically under the left kidney capsule of normoglycemic or alloxan-induced diabetic recipients 4 wk before the perfusion. In both mouse strains, islets grafted to normoglycemic animals showed an immediate distinct peak of insulin release when challenged with high glucose, whereas no response was observed from islets grafted to hyperglycemic mice. In a similar way in C57BL/KS mice, arginine stimulated insulin release from the islet grafts in normoglycemic but not in hyperglycemic recipients. Insulin treatment of the diabetic recipients, however, partially normalized the insulin response to glucose. Islet grafts were removed in toto and analyzed for contents of insulin, glucagon, somatostatin, and DNA or rates of glucose-stimulated (pro)insulin biosynthesis. In both mouse strains, islets implanted into hyperglycemic animals contained significantly less insulin, and their rates of (pro)insulin biosynthesis were markedly decreased. Insulin treatment only marginally affected these parameters. The glucagon content of the grafted islets was unaffected by the hyperglycemia in both strains of mice, whereas a significant decrease in the somatostatin content was observed in the C57BL/Ks mice. We concluded that grafted islets exposed to prolonged hyperglycemic stress become functionally impaired in mice of both strains. Our perfusion technique of islet-graft-bearing kidneys in combination with biochemical studies on the removed grafts provides a suitable model for studies of the effects of prolonged hyperglycemia on islet β-cell function
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Original Articles|
April 01 1989
Effects of Hyperglycemia on Function of Isolated Mouse Pancreatic Islets Transplanted Under Kidney Capsule
Olle Korsgren;
Olle Korsgren
Department of Medical Cell Biology, Uppsala University
Uppsala, Sweden
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Leif Jansson;
Leif Jansson
Department of Medical Cell Biology, Uppsala University
Uppsala, Sweden
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Arne Andersson
Arne Andersson
Department of Medical Cell Biology, Uppsala University
Uppsala, Sweden
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Address correspondence and reprint requests to Dr. Olle Korsgren, Department of Medical Cell Biology, Biomedical Center, P.O. Box 571, S-751 23 Uppsala, Sweden.
Diabetes 1989;38(4):510–515
Article history
Received:
August 29 1988
Revision Received:
October 19 1988
Accepted:
October 19 1988
PubMed:
2494093
Citation
Olle Korsgren, Leif Jansson, Arne Andersson; Effects of Hyperglycemia on Function of Isolated Mouse Pancreatic Islets Transplanted Under Kidney Capsule. Diabetes 1 April 1989; 38 (4): 510–515. https://doi.org/10.2337/diab.38.4.510
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