We present a new technique for analyzing the three-dimensional structure of intact isolated islets of Langerhans. Adult rat and human islets were stained with whole-mount immunofluorescence techniques and optically sectioned with a confocal microscope. This has several advantages over traditional methods: 1) the technical difficulties in serial sectioning and handling the large numbers of sections are avoided, 2) optical sectioning by confocal microscopy gives improved resolution and strongly suppresses light from out-of-focus structures, and 3) entire islets can be rapidly imaged for the presence of positive staining. This new technique should facilitate the study of the three-dimensional structure of islets of Langerhans.
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Rapid Publications| June 01 1989
Three-Dimensional Imaging of Intact Isolated Islets of Langerhans With Confocal Microscopy
T Clark Brelje;
David W Scharp;
Robert L Sorenson
Address correspondence and reprint requests to Dr. Robert L. Sorenson, Department of Cell Biology and Neuroanatomy, University of Minnesota, 321 Church Street, SE, Minneapolis, MN 55455.
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T Clark Brelje, David W Scharp, Robert L Sorenson; Three-Dimensional Imaging of Intact Isolated Islets of Langerhans With Confocal Microscopy. Diabetes 1 June 1989; 38 (6): 808–814. https://doi.org/10.2337/diab.38.6.808
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