We investigated the effectiveness of islet transplantation as therapy in an animal model of spontaneous type I (insulin-dependent) diabetes mellitus. Grafting MHC-matched and -mismatched islets with the spontaneously diabetic BB rat as a model has been previously reported to result in recurrence of the disease in the grafted tissue. When transplanted with nonimmunogenic islets isolated by nonenzymatic culture, we found that MHC-matched grafts proved to be susceptible to disease recurrence when allowed to remain in situ until ketosis developed in the host. Conversely, the MHC-mismatched grafts did not succumb to the disease process despite the destruction of the β-cell population of the endogenous pancreas. Four manifestly hyperglycemie BB/Wor rats received sufficient islet mass by allotransplantation to reverse this state. All four animals had ameliorated conditions, and three of the four were restored to a normoglycemic state. Recurrence of diabetes in the BB rat was not observed.
Islet Transplantation in Spontaneously Diabetic BB/Wor Rats
Orion D Hegre, Albert J Enriquez, Robert J Ketchum, Anthony J Weinhaus, Janet R Serie; Islet Transplantation in Spontaneously Diabetic BB/Wor Rats. Diabetes 1 September 1989; 38 (9): 1148–1154. https://doi.org/10.2337/diab.38.9.1148
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