To establish methods for stimulation of the growth and differentiation of fetal endocrine pancreatic cells, a technique for the in vitro production of fetal porcine isletlike cell clusters (ICCs) was used. By varying the composition of the culture medium with different glucose concentrations and the addition to the culture medium of insulin, growth hormone (GH), amino acids, or nicotinamide, we estimated the formation of ICCs and their hormone content. High glucose content (28.0 mM) stimulated the formation of abundant ICCs that contained decreased amounts of insulin. In contrast, culture at a low (5.6-mM) glucose concentration increased the ICC insulin content but decreased the number of ICCs formed. Addition of seven times the normal amount of amino acids hampered both the formation of ICCs and their insulin content. Neither insulin nor GH supplementation of the medium influenced the ICC insulin content, but GH stimulated an abundant outgrowth of ICCs containing relatively high insulin concentrations. However, ICCs formed under these circumstances contained <10% of the insulin content of adult islets, and further work has to be carried out to identify factors responsible for further differentiation of the fetal porcine pancreas.
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Xenogeneic Islet Preparation and Transplantation|
January 01 1989
Effects of Culture Conditions on Formation and Hormone Content of Fetal Porcine Isletlike Cell Clusters
Olle Korsgren;
Olle Korsgren
Department of Medical Cell Biology, Uppsala University
Uppsala
; Transplantation Surgery, Huddinge Hospital
Huddinge, Sweden
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Stellan Sandler;
Stellan Sandler
Department of Medical Cell Biology, Uppsala University
Uppsala
; Transplantation Surgery, Huddinge Hospital
Huddinge, Sweden
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Leif Jansson;
Leif Jansson
Department of Medical Cell Biology, Uppsala University
Uppsala
; Transplantation Surgery, Huddinge Hospital
Huddinge, Sweden
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Carl-Gustav Groth;
Carl-Gustav Groth
Department of Medical Cell Biology, Uppsala University
Uppsala
; Transplantation Surgery, Huddinge Hospital
Huddinge, Sweden
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Claes Hellerström;
Claes Hellerström
Department of Medical Cell Biology, Uppsala University
Uppsala
; Transplantation Surgery, Huddinge Hospital
Huddinge, Sweden
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Arne Andersson
Arne Andersson
Department of Medical Cell Biology, Uppsala University
Uppsala
; Transplantation Surgery, Huddinge Hospital
Huddinge, Sweden
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Address correspondence and reprint requests to Dr. Olle Korsgren, Department of Medical Cell Biology, P.O. Box 571, S-751 23 Uppsala, Sweden
Diabetes 1989;38(Supplement_1):209–212
Citation
Olle Korsgren, Stellan Sandler, Leif Jansson, Carl-Gustav Groth, Claes Hellerström, Arne Andersson; Effects of Culture Conditions on Formation and Hormone Content of Fetal Porcine Isletlike Cell Clusters. Diabetes 1 January 1989; 38 (Supplement_1): 209–212. https://doi.org/10.2337/diab.38.1.S209
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