In the presence of fixed basal levels of insulin, the route of intravenous glucose delivery (portal vs. peripheral) determines whether net hepatic glucose uptake (NHGU) occurs. Our aims were to determine if the route of intravenous glucose delivery also plays a role in regulating NHGU in the presence of hyperinsulinemia and to determine if length of fast (18 vs. 36 h) influences regulation of NHGU. Five conscious dogs fasted 18 h were given somatostatin and replacement insulin (245 ± 34 μU · kg−1 · min−1) and glucagon (0.65 ng · kg−1 · min−1) infusions intraportally. After a 40-min control period, the insulin infusion rate was increased fourfold, and glucose was infused for 3 h. Glucose was given either through a peripheral vein or the portal vein for 90 min to double the glucose load reaching the liver. The order of infusions was randomized. NHGU was measured with the arterial – venous difference technique. Insulin and glucagon levels were 12 ± 2, 35 ± 6, and 36 ± 5 μU/ml and 55 ± 12, 61 ± 13, and 59 ± 7 pg/ml during the control, peripheral, and portal infusions, respectively. The glucose infusion rate, the load of glucose reaching the liver, and the arterial-portal plasma glucose gradient were 0, 9.58 ± 2.28, and 10.44 ± 2.94 mg · kg−1 · min−1; 29.4 ± 3.6, 56.8 ± 3.4, and 56.8 ± 2.8 mg · kg−1 · min−1; and 2 ± 1, 5 ± 1, and −51 ± 15 mg/dl during the same periods. The liver switched from net glucose output (2.5 ± 0.4 mg · kg−1 · min−1) to uptake of 1.4 ± 0.7 and 3.5 ± 0.8 mg · kg−1 · min−1 during peripheral and portal glucose delivery, respectively. Despite a similar hormonal milieu and indistinguishable glucose loads, there was significantly (P < 0.01) more NHGU when glucose was infused intraportally. Identical studies in five conscious dogs fasted 36 h gave results similar to those of dogs fasted 18 h. After 36 h of fasting, NHGU was 1.6 ± 0.4 and 4.0 ± 0.4 mg · kg−1 · min−1 during the peripheral and portal glucose infusions, respectively (P < 0.005). In conclusion, the route of intravenous glucose administration plays an important role in regulating NHGU even in the presence of hyperinsulinemia in conscious dogs fasted 18 and 36 h.
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Original Articles|
January 01 1990
Interaction Between Insulin and Glucose-Delivery Route in Regulation of Net Hepatic Glucose Uptake in Conscious Dogs
Bess A Adkins-Marshall;
Bess A Adkins-Marshall
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine
Nashville, Tennessee
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Sharon R Myers;
Sharon R Myers
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine
Nashville, Tennessee
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Grant K Hendrick;
Grant K Hendrick
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine
Nashville, Tennessee
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Phil E Williams;
Phil E Williams
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine
Nashville, Tennessee
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Keith Triebwasser;
Keith Triebwasser
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine
Nashville, Tennessee
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Ben Floyd;
Ben Floyd
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine
Nashville, Tennessee
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Alan D Cherrington
Alan D Cherrington
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine
Nashville, Tennessee
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Address correspondence and reprint requests to Alan D. Cherrington, PhD, Department of Molecular Physiology and Biophysics, 702 Light Hall, Vanderbilt University School of Medicine, Nashville, TN 37232-0615.
Diabetes 1990;39(1):87–95
Article history
Received:
June 07 1988
Revision Received:
September 12 1989
Accepted:
September 12 1989
PubMed:
2210065
Citation
Bess A Adkins-Marshall, Sharon R Myers, Grant K Hendrick, Phil E Williams, Keith Triebwasser, Ben Floyd, Alan D Cherrington; Interaction Between Insulin and Glucose-Delivery Route in Regulation of Net Hepatic Glucose Uptake in Conscious Dogs. Diabetes 1 January 1990; 39 (1): 87–95. https://doi.org/10.2337/diacare.39.1.87
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