Repeated large-volume blood withdrawals started at a young age previously appeared to correct lymphopenia and prevent diabetes in Ottawa diabetes-prone BB (BBdp) rats. Therefore, we sought an early effect 24 h after withdrawal of 25% of estimated blood volume and then reexamined the long-term effects in BBdp rats. The reexamination was prompted by the occurrence of variable numbers of BBdp rats positive for RT6.1 (a T-lymphocyte differentiation alloantigen) whose presence appears to “protect” against diabetes development (identified as BBp rats). Four groups were studied: non–diabetes-prone (BBn), RT6.1− BBdp, RT6.1+ BBp, and acutely diabetic BB (BBd) rats. An acute increase in the number of peripheral blood mononuclear cells and many subsets occurred in BBd and BBp rats. Despite these acute effects, a long-term effect of repeated blood withdrawal was not found in circulating cell counts or prevention of diabetes in BBdp rats. Thus, the previous finding was probably attributable to the presence of BBp rats. The long-term study demonstrated that RT6.1 expression in BBn rats increased from low levels at 15 days, peaked at 50 days, and decreased thereafter, an important finding in interpreting RT6.1 status at different ages. Furthermore, in contrast with other subsets, MARK-1+B lymphocytes and 0X42+ monocytes/macrophages decreased markedly in number at 120 and 150 days in BBn and BBp rats, whereas counts were higher and sustained in BBdp rats. The latter finding could be related to BBdp rats successfully resisting the autoaggressive process beyond the peak age of diabetes onset.
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Original Articles|
September 01 1990
Effects of Single and Repeated Blood Withdrawals on Circulating Mononuclear Cells in BB Rats: Failure to Prevent Diabetes Despite Acute Changes in Counts
Errol B Marliss;
Errol B Marliss
McGill Nutrition and Food Science Centre, Royal Victoria Hospital
Montreal, Quebec, Canada
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Marie-Dominique Métroz-Dayer;
Marie-Dominique Métroz-Dayer
McGill Nutrition and Food Science Centre, Royal Victoria Hospital
Montreal, Quebec, Canada
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Marie Montambault;
Marie Montambault
McGill Nutrition and Food Science Centre, Royal Victoria Hospital
Montreal, Quebec, Canada
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Madeleine Faucher;
Madeleine Faucher
McGill Nutrition and Food Science Centre, Royal Victoria Hospital
Montreal, Quebec, Canada
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Mark Grose
Mark Grose
McGill Nutrition and Food Science Centre, Royal Victoria Hospital
Montreal, Quebec, Canada
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Address correspondence and reprint requests to Dr. E.B. Marliss, McGill Nutrition and Food Science Centre, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec H3A 1A1, Canada.
Diabetes 1990;39(9):1099–1105
Article history
Received:
October 11 1989
Revision Received:
April 27 1990
Accepted:
April 27 1990
PubMed:
2384190
Citation
Errol B Marliss, Marie-Dominique Métroz-Dayer, Marie Montambault, Madeleine Faucher, Mark Grose; Effects of Single and Repeated Blood Withdrawals on Circulating Mononuclear Cells in BB Rats: Failure to Prevent Diabetes Despite Acute Changes in Counts. Diabetes 1 September 1990; 39 (9): 1099–1105. https://doi.org/10.2337/diab.39.9.1099
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